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Plasmodium falciparum Field Isolates from South America Use an Atypical Red Blood Cell Invasion Pathway Associated with Invasion Ligand Polymorphisms

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dc.contributor.author Lopez-Perez, M.
dc.contributor.author Villasis Mayuri, Elizabeth Melisa
dc.contributor.author Machado, R.L.D.
dc.contributor.author Póvoa, M.M.
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Blair, S.
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Lustigman, S.
dc.date.accessioned 2022-01-18T19:34:40Z
dc.date.available 2022-01-18T19:34:40Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/11111
dc.description.abstract Studies of Plasmodium falciparum invasion pathways in field isolates have been limited. Red blood cell (RBC) invasion is a complex process involving two invasion protein families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins, which are polymorphic and not fully characterized in field isolates. To determine the various P. falciparum invasion pathways used by parasite isolates from South America, we studied the invasion phenotypes in three regions: Colombia, Peru and Brazil. Additionally, polymorphisms in three members of the EBL (EBA-181, EBA-175 and EBL-1) and five members of the PfRh (PfRh1, PfRh2a, PfRh2b, PfRh4, PfRh5) families were determined. We found that most P. falciparum field isolates from Colombia and Peru invade RBCs through an atypical invasion pathway phenotypically characterized as resistant to all enzyme treatments (NrTrCr). Moreover, the invasion pathways and the ligand polymorphisms differed substantially among the Colombian and Brazilian isolates while the Peruvian isolates represent an amalgam of those present in the Colombian and Brazilian field isolates. The NrTrCr invasion profile was associated with the presence of the PfRh2a pepC variant, the PfRh5 variant 1 and EBA-181 RVNKN variant. The ebl and Pfrh expression levels in a field isolate displaying the NrTrCr profile also pointed to PfRh2a, PfRh5 and EBA-181 as being possibly the major players in this invasion pathway. Notably, our studies demonstrate the uniqueness of the Peruvian P. falciparum field isolates in terms of their invasion profiles and ligand polymorphisms, and present a unique opportunity for studying the ability of P. falciparum parasites to expand their invasion repertoire after being reintroduced to human populations. The present study is directly relevant to asexual blood stage vaccine design focused on invasion pathway proteins, suggesting that regional invasion variants and global geographical variation are likely to preclude a simple one size fits all type of vaccine. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Animals en_US
dc.subject non phenotype en_US
dc.subject protein expression en_US
dc.subject Brazil en_US
dc.subject unclassified drug en_US
dc.subject Plasmodium falciparum en_US
dc.subject South America en_US
dc.subject Colombia en_US
dc.subject Malaria Vaccines en_US
dc.subject genetic polymorphism en_US
dc.subject Malaria, Falciparum en_US
dc.subject Polymorphism, Genetic en_US
dc.subject Protozoan Proteins en_US
dc.subject sialidase en_US
dc.subject erythrocyte en_US
dc.subject parasite isolation en_US
dc.subject Phenotype en_US
dc.subject Erythrocytes en_US
dc.subject membrane protein en_US
dc.subject Ligands en_US
dc.subject trypsin en_US
dc.subject cell invasion en_US
dc.subject chymotrypsin en_US
dc.subject erythrocyte antigen en_US
dc.subject protein EBA 175 en_US
dc.subject protein EBA 181 en_US
dc.subject protein EBL 1 en_US
dc.subject protein family en_US
dc.subject protein PfRh1 en_US
dc.subject protein PfRh2a en_US
dc.subject protein PfRh2b en_US
dc.subject protein PfRh4 en_US
dc.subject protein PfRh5 en_US
dc.subject protein variant en_US
dc.subject Reticulocytes en_US
dc.title Plasmodium falciparum Field Isolates from South America Use an Atypical Red Blood Cell Invasion Pathway Associated with Invasion Ligand Polymorphisms en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0047913
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.00
dc.relation.issn 1932-6203


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