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Genetic Diversity and Transmission Characteristics of Beijing Family Strains of Mycobacterium tuberculosis in Peru

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dc.contributor.author Iwamoto, T.
dc.contributor.author Grandjean, Louis
dc.contributor.author Arikawa, K.
dc.contributor.author Nakanishi, N.
dc.contributor.author Caviedes, L.
dc.contributor.author Coronel, J.
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Wada, T.
dc.contributor.author Taype, C.A.
dc.contributor.author Shaw, M.-A.
dc.contributor.author Moore, David Alexander James
dc.contributor.author Gilman, Robert Hugh
dc.date.accessioned 2022-01-18T19:34:40Z
dc.date.available 2022-01-18T19:34:40Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/11112
dc.description.abstract Beijing family strains of Mycobacterium tuberculosis have attracted worldwide attention because of their wide geographical distribution and global emergence. Peru, which has a historical relationship with East Asia, is considered to be a hotspot for Beijing family strains in South America. We aimed to unveil the genetic diversity and transmission characteristics of the Beijing strains in Peru. A total of 200 Beijing family strains were identified from 2140 M. tuberculosis isolates obtained in Lima, Peru, between December 2008 and January . Of them, 198 strains were classified into sublineages, on the basis of 10 sets of single nucleotide polymorphisms (SNPs). They were also subjected to variable number tandem-repeat (VNTR) typing using an international standard set of 15 loci (15-MIRU-VNTR) plus 9 additional loci optimized for Beijing strains. An additional 70 Beijing family strains, isolated between 1999 and 2006 in Lima, were also analyzed in order to make a longitudinal comparison. The Beijing family was the third largest spoligotyping clade in Peru. Its population structure, by SNP typing, was characterized by a high frequency of Sequence Type 10 (ST10), which belongs to a modern subfamily of Beijing strains (178/198, 89.9%). Twelve strains belonged to the ancient subfamily (ST3 [n = 3], ST25 [n = 1], ST19 [n = 8]). Overall, the polymorphic information content for each of the 24 loci values was low. The 24 loci VNTR showed a high clustering rate (80.3%) and a high recent transmission index (RTIn-1 = 0.707). These strongly suggest the active and on-going transmission of Beijing family strains in the survey area. Notably, 1 VNTR genotype was found to account for 43.9% of the strains. Comparisons with data from East Asia suggested the genotype emerged as a uniquely endemic clone in Peru. A longitudinal comparison revealed the genotype was present in Lima by 1999. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject major clinical study en_US
dc.subject Genotype en_US
dc.subject geographic distribution en_US
dc.subject Prevalence en_US
dc.subject Genetic Variation en_US
dc.subject China en_US
dc.subject Polymorphism, Single Nucleotide en_US
dc.subject gene frequency en_US
dc.subject Models, Genetic en_US
dc.subject population structure en_US
dc.subject longitudinal study en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Tuberculosis en_US
dc.subject bacterium identification en_US
dc.subject gene locus en_US
dc.subject bacterium isolate en_US
dc.subject molecular typing en_US
dc.subject genetic variability en_US
dc.subject bacterial strain en_US
dc.subject Epidemics en_US
dc.subject variable number of tandem repeat en_US
dc.subject gene cluster en_US
dc.subject bacterial genetics en_US
dc.subject bacterium examination en_US
dc.subject bacterial transmission en_US
dc.subject Alleles en_US
dc.subject cladistics en_US
dc.subject genetic line en_US
dc.subject species comparison en_US
dc.title Genetic Diversity and Transmission Characteristics of Beijing Family Strains of Mycobacterium tuberculosis in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0049651
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.00
dc.relation.issn 1932-6203


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