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dc.contributor.author | Iwamoto, T. | |
dc.contributor.author | Grandjean, Louis | |
dc.contributor.author | Arikawa, K. | |
dc.contributor.author | Nakanishi, N. | |
dc.contributor.author | Caviedes, L. | |
dc.contributor.author | Coronel, J. | |
dc.contributor.author | Sheen Cortavarria, Patricia | |
dc.contributor.author | Wada, T. | |
dc.contributor.author | Taype, C.A. | |
dc.contributor.author | Shaw, M.-A. | |
dc.contributor.author | Moore, David Alexander James | |
dc.contributor.author | Gilman, Robert Hugh | |
dc.date.accessioned | 2022-01-18T19:34:40Z | |
dc.date.available | 2022-01-18T19:34:40Z | |
dc.date.issued | 2012 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/11112 | |
dc.description.abstract | Beijing family strains of Mycobacterium tuberculosis have attracted worldwide attention because of their wide geographical distribution and global emergence. Peru, which has a historical relationship with East Asia, is considered to be a hotspot for Beijing family strains in South America. We aimed to unveil the genetic diversity and transmission characteristics of the Beijing strains in Peru. A total of 200 Beijing family strains were identified from 2140 M. tuberculosis isolates obtained in Lima, Peru, between December 2008 and January . Of them, 198 strains were classified into sublineages, on the basis of 10 sets of single nucleotide polymorphisms (SNPs). They were also subjected to variable number tandem-repeat (VNTR) typing using an international standard set of 15 loci (15-MIRU-VNTR) plus 9 additional loci optimized for Beijing strains. An additional 70 Beijing family strains, isolated between 1999 and 2006 in Lima, were also analyzed in order to make a longitudinal comparison. The Beijing family was the third largest spoligotyping clade in Peru. Its population structure, by SNP typing, was characterized by a high frequency of Sequence Type 10 (ST10), which belongs to a modern subfamily of Beijing strains (178/198, 89.9%). Twelve strains belonged to the ancient subfamily (ST3 [n = 3], ST25 [n = 1], ST19 [n = 8]). Overall, the polymorphic information content for each of the 24 loci values was low. The 24 loci VNTR showed a high clustering rate (80.3%) and a high recent transmission index (RTIn-1 = 0.707). These strongly suggest the active and on-going transmission of Beijing family strains in the survey area. Notably, 1 VNTR genotype was found to account for 43.9% of the strains. Comparisons with data from East Asia suggested the genotype emerged as a uniquely endemic clone in Peru. A longitudinal comparison revealed the genotype was present in Lima by 1999. | en_US |
dc.language.iso | eng | |
dc.publisher | Public Library of Science | |
dc.relation.ispartofseries | PLoS ONE | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | Humans | en_US |
dc.subject | Peru | en_US |
dc.subject | major clinical study | en_US |
dc.subject | Genotype | en_US |
dc.subject | geographic distribution | en_US |
dc.subject | Prevalence | en_US |
dc.subject | Genetic Variation | en_US |
dc.subject | China | en_US |
dc.subject | Polymorphism, Single Nucleotide | en_US |
dc.subject | gene frequency | en_US |
dc.subject | Models, Genetic | en_US |
dc.subject | population structure | en_US |
dc.subject | longitudinal study | en_US |
dc.subject | Mycobacterium tuberculosis | en_US |
dc.subject | Tuberculosis | en_US |
dc.subject | bacterium identification | en_US |
dc.subject | gene locus | en_US |
dc.subject | bacterium isolate | en_US |
dc.subject | molecular typing | en_US |
dc.subject | genetic variability | en_US |
dc.subject | bacterial strain | en_US |
dc.subject | Epidemics | en_US |
dc.subject | variable number of tandem repeat | en_US |
dc.subject | gene cluster | en_US |
dc.subject | bacterial genetics | en_US |
dc.subject | bacterium examination | en_US |
dc.subject | bacterial transmission | en_US |
dc.subject | Alleles | en_US |
dc.subject | cladistics | en_US |
dc.subject | genetic line | en_US |
dc.subject | species comparison | en_US |
dc.title | Genetic Diversity and Transmission Characteristics of Beijing Family Strains of Mycobacterium tuberculosis in Peru | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0049651 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.01.00 | |
dc.relation.issn | 1932-6203 |
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