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Mechanisms of liver fibrosis associated with experimental fasciola hepatica infection: Roles of Fas2 proteinase and hepatic stellate cell activation

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dc.contributor.author Marcos, L.A.
dc.contributor.author Terashima, A.
dc.contributor.author Yi, P.
dc.contributor.author Andrade, R.
dc.contributor.author Cubero, F.J.
dc.contributor.author Albanis, E.
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Espinoza, J.R.
dc.contributor.author Friedman, S.L.
dc.date.accessioned 2022-01-18T19:34:43Z
dc.date.available 2022-01-18T19:34:43Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/11191
dc.description.abstract We have evaluated the possible mechanisms of liver fibrosis caused by Fasciola hepatica in an animal model and in culture using immortalized human stellate cells. Liver biopsies of F. hepatica-infected rats were performed at wk 8 and 16. Serum-starved LX-2 cells, a human stellate cell line, were exposed to increasing concentrations of Fas2 antigen. The expression of key fibrosis-related genes was evaluated by qRT-PCR. There was a significant correlation between fibrogenic gene expression and both intensity and duration of infection. LX-2 cells exposed to Fas2 showed progressively increased expression of mRNAs for Collagen I, alpha-smooth muscle-actin, platelet-derived growth factor beta receptor, and tissue inhibitor of metalloproteinase II; inhibition of Fas2 cysteine proteinase activity by E-64 abrogated these increases, suggesting that the protease activity of Fas2 is involved in fibrogenic stimulation. In summary, F. hepatica infection is associated with up-regulation of mRNAs associated with hepatic fibrogenesis in vivo and in activated hepatic stellate cells. en_US
dc.language.iso eng
dc.publisher American Society of Parasitologists
dc.relation.ispartofseries Journal of Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans|controlled study en_US
dc.subject pathology en_US
dc.subject animal en_US
dc.subject animal experiment en_US
dc.subject animal model en_US
dc.subject animal tissue en_US
dc.subject Animals en_US
dc.subject collagen type 1 en_US
dc.subject genetics en_US
dc.subject in vitro study en_US
dc.subject in vivo study en_US
dc.subject metabolism en_US
dc.subject non|physiology en_US
dc.subject pathogenicity en_US
dc.subject polymerase chain reaction en_US
dc.subject infectious disease en_US
dc.subject parasite antigen en_US
dc.subject unclassified drug en_US
dc.subject disease association en_US
dc.subject gene expression en_US
dc.subject Disease Models, Animal en_US
dc.subject Analysis of Variance en_US
dc.subject Cell Line en_US
dc.subject messenger RNA en_US
dc.subject upregulation en_US
dc.subject disease model en_US
dc.subject real time polymerase chain reaction en_US
dc.subject enzymology en_US
dc.subject tissue inhibitor of metalloproteinase 1 en_US
dc.subject Fasciola hepatica en_US
dc.subject Fascioliasis en_US
dc.subject enzyme activity en_US
dc.subject Animalia en_US
dc.subject Antigens, Helminth en_US
dc.subject cysteine proteinase en_US
dc.subject Rats en_US
dc.subject analysis of variance en_US
dc.subject Gene Expression en_US
dc.subject functional morphology en_US
dc.subject genetic analysis en_US
dc.subject numerical model en_US
dc.subject gelatinase A en_US
dc.subject experimental study en_US
dc.subject transforming growth factor beta1 en_US
dc.subject Rattus en_US
dc.subject Actins en_US
dc.subject alpha smooth muscle actin en_US
dc.subject antigen en_US
dc.subject cell activation en_US
dc.subject cell organelle en_US
dc.subject Collagen en_US
dc.subject Cysteine Endopeptidases en_US
dc.subject Fas antigen en_US
dc.subject Fas2 antigen en_US
dc.subject Fas2 antigen, Fasciola hepatica en_US
dc.subject fibrogenesis en_US
dc.subject flatworm en_US
dc.subject Hepatic Stellate Cells en_US
dc.subject inhibitor en_US
dc.subject liver biopsy en_US
dc.subject Liver Cirrhosis en_US
dc.subject liver fibrosis en_US
dc.subject n [n (3 carboxyoxirane 2 carbonyl)leucyl]agmatine en_US
dc.subject platelet derived growth factor beta receptor en_US
dc.subject Receptor, Platelet-Derived Growth Factor beta en_US
dc.subject stellate cell en_US
dc.subject tissue inhibitor of metalloproteinase 2 en_US
dc.subject Tissue Inhibitor of Metalloproteinase-2 en_US
dc.title Mechanisms of liver fibrosis associated with experimental fasciola hepatica infection: Roles of Fas2 proteinase and hepatic stellate cell activation en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1645/GE-2420.1
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1937-2345


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