Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA

Lin, S.; García Lescano, Héctor Hugo; Gilman, Robert Hugh; Rodriguez, S.; Tsang, V.C.W.; Handali, S.; Scheel, C.M.; Hancock, K.; Pattabhi, S.; Lee, Y.-M.; Levin, A.; Kovalenko, V.A.; Gonzalez Zariquiey, Armando Emiliano

dc.contributor.author	Lee, Y.-M.
dc.contributor.author	Handali, S.
dc.contributor.author	Hancock, K.
dc.contributor.author	Pattabhi, S.
dc.contributor.author	Kovalenko, V.A.
dc.contributor.author	Levin, A.
dc.contributor.author	Rodriguez, S.
dc.contributor.author	Lin, S.
dc.contributor.author	Scheel, C.M.
dc.contributor.author	Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Tsang, V.C.W.
dc.date.accessioned	2022-01-18T19:35:51Z
dc.date.available	2022-01-18T19:35:51Z
dc.date.issued	2011
dc.identifier.uri	https://hdl.handle.net/20.500.12866/11212
dc.description.abstract	Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T 24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	controlled study	en_US
dc.subject	animal cell	en_US
dc.subject	Animals	en_US
dc.subject	non nuclear magnetic resonance imaging	en_US
dc.subject	parasitology	en_US
dc.subject	parasite antigen	en_US
dc.subject	unclassified drug	en_US
dc.subject	methodology	en_US
dc.subject	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject	Sensitivity and Specificity	en_US
dc.subject	immunology	en_US
dc.subject	diagnostic test accuracy study	en_US
dc.subject	Serologic Tests	en_US
dc.subject	serology	en_US
dc.subject	computer assisted tomography	en_US
dc.subject	cyst (resting stage)	en_US
dc.subject	enzyme linked immunosorbent assay	en_US
dc.subject	Taenia solium	en_US
dc.subject	Cysticercosis	en_US
dc.subject	antibody detection	en_US
dc.subject	Antigens, Helminth	en_US
dc.subject	autoanalyzer	en_US
dc.subject	baculovirus expression system	en_US
dc.subject	enzyme linked immunoelectrotransfer blot	en_US
dc.subject	glycoprotein	en_US
dc.subject	helminth antibody	en_US
dc.subject	helminth protein	en_US
dc.subject	horseradish peroxidase	en_US
dc.subject	immunoassay	en_US
dc.subject	Lens culinaris	en_US
dc.subject	lentil lectin purified glycoprotein	en_US
dc.subject	quantitative assay	en_US
dc.subject	quick enzyme linked immunosorbent assay	en_US
dc.subject	recombinant antigen	en_US
dc.subject	recombinant antigen gp 50	en_US
dc.subject	recombinant antigen sts 18 var1	en_US
dc.subject	recombinant antigen t 24h	en_US
dc.subject	serodiagnosis	en_US
dc.subject	serum	en_US
dc.subject	streptavidin	en_US
dc.title	Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.2011.10-0079
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645