Universidad Peruana Cayetano Heredia

Polymerase chain reaction detection of leishmania kDNA from the urine of Peruvian patients with cutaneous and mucocutaneous leishmaniasis

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dc.contributor.author Veland, N.
dc.contributor.author Espinosa, D.
dc.contributor.author Valencia, B.M.
dc.contributor.author Ramos, A.P.
dc.contributor.author Calderon, F.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Low, D.E.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Boggild, A.K.
dc.date.accessioned 2022-01-18T19:35:51Z
dc.date.available 2022-01-18T19:35:51Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/11213
dc.description.abstract We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase chain reaction (PCR) was performed on samples from patients and 10 healthy volunteers from non-endemic areas. Eighty-six of 108 patients were diagnosed with CL and 18 (21%) had detectable Leishmania Viannia kDNA in the urine. Sensitivity and specificity were 20.9% (95% confidence interval [CI] 12.3-29.5%) and 100%. Six of 8 patients with mucocutaneous involvement had detectable kDNA in urine versus 12 of 78 patients with isolated cutaneous disease (P < 0.001). L. (V.) braziliensis (N = 3), L. (V.) guyanensis (N = 6), and L. (V.) peruviana (N = 3) were identified from urine. No healthy volunteer or patient with an alternate diagnosis had detectable kDNA in urine. Sensitivity of urine PCR is sub-optimal for diagnosis. On the basis of these preliminary data in a small number of patients, detectable kDNA in urine may identify less localized forms of infection and inform treatment decisions. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject controlled study en_US
dc.subject isolation and purification en_US
dc.subject major clinical study en_US
dc.subject genetics en_US
dc.subject non parasitology en_US
dc.subject polymerase chain reaction en_US
dc.subject unclassified drug en_US
dc.subject school child en_US
dc.subject Leishmania braziliensis en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.subject skin leishmaniasis en_US
dc.subject kinetoplast DNA en_US
dc.subject parasite identification en_US
dc.subject diagnostic test accuracy study en_US
dc.subject sensitivity and specificity en_US
dc.subject restriction fragment length polymorphism en_US
dc.subject Leishmania en_US
dc.subject protozoal DNA en_US
dc.subject protozoal protein en_US
dc.subject DNA, Kinetoplast en_US
dc.subject aspiration en_US
dc.subject cysteine proteinase en_US
dc.subject cysteine proteinase b en_US
dc.subject diagnostic kit en_US
dc.subject heat shock protein 70 en_US
dc.subject Leishmania guyanensis en_US
dc.subject Leishmania peruviana en_US
dc.subject Leishmaniasis, Mucocutaneous en_US
dc.subject mannose phosphate isomerase en_US
dc.subject predictive value en_US
dc.subject skin examination en_US
dc.subject skin test en_US
dc.subject smear en_US
dc.subject urinalysis en_US
dc.subject urine en_US
dc.subject urine culture en_US
dc.title Polymerase chain reaction detection of leishmania kDNA from the urine of Peruvian patients with cutaneous and mucocutaneous leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.2011.10-0556
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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