Lack of molecular correlates of Plasmodium vivax ookinete development

Vinetz, Joseph Michael; Neyra, V.; Bounkeua, V.; Chuquiyauri, R.; Abeles, S.R.; Llanos Cuentas, Elmer Alejandro; Yori, P.P.; McClean, C.M.; Li, F.

dc.contributor.author	Bounkeua, V.
dc.contributor.author	Li, F.
dc.contributor.author	Chuquiyauri, R.
dc.contributor.author	Abeles, S.R.
dc.contributor.author	McClean, C.M.
dc.contributor.author	Neyra, V.
dc.contributor.author	Llanos Cuentas, Elmer Alejandro
dc.contributor.author	Yori, P.P.
dc.contributor.author	Vinetz, Joseph Michael
dc.date.accessioned	2022-01-18T19:35:51Z
dc.date.available	2022-01-18T19:35:51Z
dc.date.issued	2011
dc.identifier.uri	https://hdl.handle.net/20.500.12866/11214
dc.description.abstract	Previous studies of Plasmodium vivax transmission to Anopheles spp. mosquitoes have not been able to predict mosquito infectivity on the basis of microscopic or molecular quantification of parasites (total parasites in the sample or total number of gametocytes) in infected blood. Two methods for production of P. vivax ookinete cultures in vitro, with yields of 106 macrogametocytes, 104 zygotes, and 103 ookinetes, respectively, per 10 mL of P. vivax-infected patient blood with approximately 0.01% parasitemia, were used to study P. vivax sexual stage development. The quantity of gametocytes, determined by counting Giemsa-stained blood smears, and quantity and type of gametocyte as determined by quantitative reverse transcriptase-polymerase chain reaction for Pvalpha tubulin II and macrogametocyte-specific pvg377 did not predict ookinete yield. Factors that affect the efficiency of in vitro P. vivax ookinete transformation remain poorly understood.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	in vitro study	en_US
dc.subject	non Plasmodium vivax	en_US
dc.subject	sex ratio	en_US
dc.subject	clinical article	en_US
dc.subject	parasitemia	en_US
dc.subject	Reverse Transcriptase Polymerase Chain Reaction	en_US
dc.subject	Malaria, Vivax	en_US
dc.subject	Plasmodium vivax malaria	en_US
dc.subject	Antimalarials	en_US
dc.subject	blood smear	en_US
dc.subject	Cells, Cultured	en_US
dc.subject	gametocyte	en_US
dc.subject	maturity	en_US
dc.subject	ookinete development	en_US
dc.subject	reverse transcription polymerase chain reaction	en_US
dc.subject	sexual development	en_US
dc.subject	xanthurenic acid	en_US
dc.subject	zygote	en_US
dc.title	Lack of molecular correlates of Plasmodium vivax ookinete development	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.2011.10-0729
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645