Universidad Peruana Cayetano Heredia

Detection of AA-type amyloid protein in labial salivary glands

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dc.contributor.author Sacsaquispe, S.-J.
dc.contributor.author Antúnez-de Mayolo, E.-A.
dc.contributor.author Vicetti, R.
dc.contributor.author Delgado, W.-A.
dc.date.accessioned 2022-01-18T19:35:51Z
dc.date.available 2022-01-18T19:35:51Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/11218
dc.description.abstract Objectives: Among the diverse forms of amyloidosis, secondary type is the most frequent one. Diagnosis of amyloid deposition is based on the identification of the fibrillary protein amyloid by means of Congo Red (CR) or crystal violet (CV) stains, but these techniques do not differentiate between the different types of amyloid fibrils. The aim of this study was to identify by immunofluorescence (IF) AA amyloid a pathological fibrillar low-molecularweight protein formed by cleavage of serum amyloid A (SAA) protein in labial salivary gland (LSG) biopsies from patients with secondary amyloidosis. Study design: 98 LSG were studied, 65 were from patients with secondary amyloidosis and 33 from subjects with chronic inflammatory diseases without evidence of this anomaly. All sections were stained with hematoxylin and eosin (H&E), CV, CR and IF using anti-AA antibodies. Positive and negative controls were used for all techniques. Results: CV and CR demonstrated that the amyloid substance was found mainly distributed periductally (93.8%), followed by periacinar and perivascular locations (p<0.001); however, the IF demonstrated that amyloid AA substance predominates in the periacinar area (73.8%), followed by periductal and perivascular locations (p<0.001). IF has a sensitivity of 83%, 100% of specificity, 100% of predictive positive value and 75% of predictive negative value.Conclusions: The results of this study confirm the efficacy of the LSG biopsy as a highly reliable method for diagnosis of secondary amyloidosis. en_US
dc.language.iso eng
dc.publisher Medicina Oral
dc.relation.ispartofseries Medicina Oral, Patología Oral y Cirugía Bucal
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject major clinical study en_US
dc.subject human tissue en_US
dc.subject Sensitivity and Specificity en_US
dc.subject eosin en_US
dc.subject hematoxylin en_US
dc.subject protein analysis en_US
dc.subject Amyloidosis en_US
dc.subject Fluorescent Antibody Technique en_US
dc.subject protein localization en_US
dc.subject chronic inflammation en_US
dc.subject molecular weight en_US
dc.subject Lip en_US
dc.subject Amyloid en_US
dc.subject amyloid A protein en_US
dc.subject amyloidosis en_US
dc.subject Immunofluorescence en_US
dc.subject protein cleavage en_US
dc.subject Salivary Gland Diseases en_US
dc.subject Salivary glands en_US
dc.subject Serum Amyloid A Protein en_US
dc.title Detection of AA-type amyloid protein in labial salivary glands en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4317/medoral.16.e149
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.14
dc.relation.issn 1698-6946


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