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Improved diagnosis of viable parenchymal neurocysticercosis by combining antibody banding patterns on enzyme-linked immunoelectrotransfer blot (EITB) with antigen ELISA assay.

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dc.contributor.author Arroyo Hurtado, Gianfranco
dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author Lescano Guevara, Andres Guillermo
dc.contributor.author Gonzales, Isidro
dc.contributor.author Saavedra Pastor, Herbert
dc.contributor.author Pretell, E. Javier
dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Perez, Erika
dc.contributor.author Handali, Sukwan
dc.contributor.author Noh, John
dc.contributor.author Dorny, Pierre
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author O'Neal, Seth E.
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author García Lescano, Héctor Hugo
dc.date.accessioned 2022-02-01T21:18:26Z
dc.date.available 2022-02-01T21:18:26Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/11266
dc.description.abstract The diagnosis of neurocysticercosis (NCC) depends on neuroimaging and serological confirmation. While antibody detection by enzyme-linked immunoelectrotransfer blot (EITB) fails to predict viable NCC, EITB banding patterns provide information about the host's infection course. Adding antigen enzyme-linked immunosorbent assay (Ag-ELISA) results to EITB banding patterns may improve their ability to predict or rule out of viable NCC. We assessed whether combining EITB banding patterns with Ag-ELISA improves discrimination of viable infection in imaging-confirmed parenchymal NCC. EITB banding patterns were grouped into classes using latent class analysis. True-positive and false-negative Ag-ELISA results in each class were compared using Fisher's exact test. Four classes were identified: 1, EITB negative or positive to GP50 alone (GP50 antigen family); 2, positive to GP42-39 and GP24 (T24/42 family), with or without GP50; and 3 and 4, positive to GP50, GP42-39, and GP24 and reacting to bands in the 8-kDa family. Most cases in classes 3 and 4 had viable NCC (82% and 88%, respectively) compared to classes 2 and 1 (53% and 5%, respectively). Adding positive Ag-ELISA results to class 2 predicted all viable NCC cases (22/22 [100%]), whereas 11/40 patients (27.5%) Ag-ELISA negative had viable NCC (P < 0.001). Only 1/4 patients (25%) Ag-ELISA positive in class 1 had viable NCC, whereas 1/36 patients (2.8%) Ag-ELISA negative had viable NCC (P = 0.192). In classes 3 and 4, adding Ag-ELISA was not contributory. Combining Ag-ELISA with EITB banding patterns improves discrimination of viable from nonviable NCC, particularly for class 2 responses. Together, these complement neuroimaging more appropriately for the diagnosis of viable NCC. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Ag-ELISA en_US
dc.subject EITB banding patterns en_US
dc.subject Taenia solium en_US
dc.subject Viable NCC en_US
dc.title Improved diagnosis of viable parenchymal neurocysticercosis by combining antibody banding patterns on enzyme-linked immunoelectrotransfer blot (EITB) with antigen ELISA assay. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.01550-21
dc.relation.issn 1098-660X


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