Universidad Peruana Cayetano Heredia

Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression

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dc.contributor.author Blandino, Alice
dc.contributor.author Scherer, Dominique
dc.contributor.author Rounge, Trine B.
dc.contributor.author Umu, Sinan U.
dc.contributor.author Boekstegers, Felix
dc.contributor.author Barahona Ponce, Carol
dc.contributor.author Marcelain, Katherine
dc.contributor.author Gárate-Calderón, Valentina
dc.contributor.author Waldenberger, Melanie
dc.contributor.author Morales, Erik
dc.contributor.author Rojas, Armando
dc.contributor.author Munoz, César
dc.contributor.author Retamales, Javier
dc.contributor.author de Toro, Gonzalo
dc.contributor.author Barajas, Olga
dc.contributor.author Rivera, Maria Teresa
dc.contributor.author Cortés, Analía
dc.contributor.author Loader, Denisse
dc.contributor.author Saavedra, Javiera
dc.contributor.author Gutiérrez, Lorena
dc.contributor.author Ortega, Alejandro
dc.contributor.author Bertrán, Maria Enriqueta
dc.contributor.author Gabler, Fernando
dc.contributor.author Campos, Mónica
dc.contributor.author Alvarado, Juan
dc.contributor.author Moisán, Fabrizio
dc.contributor.author Spencer, Loreto
dc.contributor.author Nervi, Bruno
dc.contributor.author Carvajal-Hausdorf, Daniel E.
dc.contributor.author Losada, Héctor
dc.contributor.author Almau, Mauricio
dc.contributor.author Fernández, Plinio
dc.contributor.author Gallegos, Iván
dc.contributor.author Olloquequi, Jordi
dc.contributor.author Fuentes-Guajardo, Macarena
dc.contributor.author Gonzalez-Jose, Rolando
dc.contributor.author Bortolini, Maria Cátira
dc.contributor.author Gallo López-Aliaga, Carla Maria
dc.contributor.author Ruiz Linares, Andrés
dc.contributor.author Rothhammer, Francisco
dc.contributor.author Bermejo, Justo Lorenzo
dc.date.accessioned 2022-02-17T19:23:12Z
dc.date.available 2022-02-17T19:23:12Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/11369
dc.description.abstract Long noncoding RNAs (lncRNAs) play key roles in cell processes and are good candi-dates for cancer risk prediction. Few studies have investigated the association between individual genotypes and lncRNA expression. Here we integrate three separate datasets with information on lncRNA expression only, both lncRNA expression and genotype, and genotype information only to identify circulating lncRNAs associated with the risk of gallbladder cancer (GBC) using robust linear and logistic regression techniques. In the first dataset, we preselect lncRNAs based on expression changes along the sequence “gallstones → dysplasia → GBC”. In the second dataset, we validate associations between genetic variants and serum expression levels of the preselected lncR-NAs (cis-lncRNA-eQTLs) and build lncRNA expression prediction models. In the third dataset, we predict serum lncRNA expression based on individual genotypes and assess the association between genotype-based expression and GBC risk. AC084082.3 and LINC00662 showed increasing expression levels (p-value = 0.009), while C22orf34 expression decreased in the sequence from gallstones to GBC (p-value = 0.04). We identified and validated two cis-LINC00662-eQTLs (r2 = 0.26) and three cis-C22orf34-eQTLs (r2 = 0.24). Only LINC00662 showed a genotyped-based serum expression associated with GBC risk (OR = 1.25 per log2 expression unit, 95% CI 1.04–1.52, p-value = 0.02). Our results suggest that preselection of lncRNAs based on tissue samples and exploitation of cis-lncRNA-eQTLs may facilitate the identification of circulating noncoding RNAs linked to cancer risk. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Cancers
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Gallbladder cancer en_US
dc.subject EQTLs en_US
dc.subject Genetic association study en_US
dc.subject LncRNAs en_US
dc.subject Molecular phenotypes en_US
dc.title Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/cancers14030634
dc.relation.issn 2072-6694


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