Mostrar el registro sencillo del ítem
dc.contributor.author | Alcedo, Katherine | |
dc.contributor.author | Ruiz, Joaquim | |
dc.contributor.author | Ochoa Woodell, Theresa Jean | |
dc.contributor.author | Riveros Ramirez, Maribel Denise | |
dc.date.accessioned | 2022-03-23T16:54:20Z | |
dc.date.available | 2022-03-23T16:54:20Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/11477 | |
dc.description.abstract | Background: Antibiotic-resistant Escherichia coli can colonize the intestinal tract of healthy children, causing concern when antibiotic resistance is related to the presence of transferable mechanisms, such as extended-spectrum β-lactamases (ESBLs). Materials and Methods: Fecal samples from 41 healthy children from two villages of rural Peru were cultured on ceftriaxone-disks. ESBL production was confirmed with double disk synergy. In all ESBL-produced isolates, antibiotic susceptibility to 12 antibacterial agents was established by disk diffusion, while clonal relationships were determined by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). Presence of ST131 was determined using PCR. Results: Ceftriaxone-resistant microorganisms were recovered from 39 samples belonging to 22 out of 41 children (53.7%). Of these, 80 ceftriaxone-resistant and two ceftriaxone-intermediate E. coli from inside ceftriaxone-halos were confirmed as ESBL-producers. All isolates were multidrug-resistant. In 79/80 (98.8%) ceftriaxone-resistant isolates, the presence of blaCTX-M was detected alone (58 isolates, or together with other β-lactamase (blaTEM, 17 isolates; blaOXA-1-like, 3 isolates; blaTEM + blaOXA-1-like, 1 isolate), while in one isolate no such ESBL was identified. The two ceftriaxone-intermediate isolates recovered from the same sample, carried a blaTEM and blaSHV respectively. Thirty-four different clones were identified, with 4 clones being recovered from different samples from the same child. Twelve clones were disseminated among different children, including 5 clones disseminated between both villages. Two clones, accounting for 3 isolates and both recovered from the same children, belonged to E. coli ST131. Conclusion: This study demonstrates high prevalence of ESBL-carriers among healthy children living in a rural area of Peru, stressing the need for continuous surveillance and search for public health control measures. | en_US |
dc.language.iso | eng | |
dc.publisher | Korean Society of Infectious Diseases | |
dc.relation.ispartofseries | Infection and Chemotherapy | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | Escherichia coli | en_US |
dc.subject | Commensal | en_US |
dc.subject | Antibiotic-resistance | en_US |
dc.subject | Extended-spectrum β-lactamases | en_US |
dc.subject | Healthy carriers | en_US |
dc.title | High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from Healthy Children | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.3947/ic.2021.0102 | |
dc.relation.issn | 2093-2340 |
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a este ítem. |