Universidad Peruana Cayetano Heredia

High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from Healthy Children

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dc.contributor.author Alcedo, Katherine
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.date.accessioned 2022-03-23T16:54:20Z
dc.date.available 2022-03-23T16:54:20Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/11477
dc.description.abstract Background: Antibiotic-resistant Escherichia coli can colonize the intestinal tract of healthy children, causing concern when antibiotic resistance is related to the presence of transferable mechanisms, such as extended-spectrum β-lactamases (ESBLs). Materials and Methods: Fecal samples from 41 healthy children from two villages of rural Peru were cultured on ceftriaxone-disks. ESBL production was confirmed with double disk synergy. In all ESBL-produced isolates, antibiotic susceptibility to 12 antibacterial agents was established by disk diffusion, while clonal relationships were determined by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). Presence of ST131 was determined using PCR. Results: Ceftriaxone-resistant microorganisms were recovered from 39 samples belonging to 22 out of 41 children (53.7%). Of these, 80 ceftriaxone-resistant and two ceftriaxone-intermediate E. coli from inside ceftriaxone-halos were confirmed as ESBL-producers. All isolates were multidrug-resistant. In 79/80 (98.8%) ceftriaxone-resistant isolates, the presence of blaCTX-M was detected alone (58 isolates, or together with other β-lactamase (blaTEM, 17 isolates; blaOXA-1-like, 3 isolates; blaTEM + blaOXA-1-like, 1 isolate), while in one isolate no such ESBL was identified. The two ceftriaxone-intermediate isolates recovered from the same sample, carried a blaTEM and blaSHV respectively. Thirty-four different clones were identified, with 4 clones being recovered from different samples from the same child. Twelve clones were disseminated among different children, including 5 clones disseminated between both villages. Two clones, accounting for 3 isolates and both recovered from the same children, belonged to E. coli ST131. Conclusion: This study demonstrates high prevalence of ESBL-carriers among healthy children living in a rural area of Peru, stressing the need for continuous surveillance and search for public health control measures. en_US
dc.language.iso eng
dc.publisher Korean Society of Infectious Diseases
dc.relation.ispartofseries Infection and Chemotherapy
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Escherichia coli en_US
dc.subject Commensal en_US
dc.subject Antibiotic-resistance en_US
dc.subject Extended-spectrum β-lactamases en_US
dc.subject Healthy carriers en_US
dc.title High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from Healthy Children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3947/ic.2021.0102
dc.relation.issn 2093-2340


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