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Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior

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dc.contributor.author Alvarez, Marcus Vinicius Nis
dc.contributor.author Alonso, Diego Peres
dc.contributor.author Kadri, Samir Moura
dc.contributor.author Rufalco-Moutinho, Paulo
dc.contributor.author Bernardes, Isabella Adriadne Ferrari
dc.contributor.author de Mello, Ana Carolina Florindo
dc.contributor.author Souto, Ana Carolina
dc.contributor.author Carrasco Escobar, Gabriel
dc.contributor.author Moreno, Marta
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Conn, Jan E.
dc.contributor.author Ribolla, Paulo E.M.
dc.date.accessioned 2022-06-01T13:53:55Z
dc.date.available 2022-06-01T13:53:55Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/11709
dc.description.abstract Background: In Brazil, malaria is concentrated in the Amazon Basin, where more than 99% of the annual cases are reported. The main goal of this study was to investigate the population structure and genetic association of the biting behavior of Nyssorhynchus (also known as Anopheles) darlingi, the major malaria vector in the Amazon region of Brazil, using low-coverage genomic sequencing data. Methods: Samples were collected in the municipality of Mâncio Lima, Acre state, Brazil between 2016 and 2017. Different approaches using genotype imputation and no gene imputation for data treatment and low-coverage sequencing genotyping were performed. After the samples were genotyped, population stratification analysis was performed. Results: Weak but statistically significant stratification signatures were identified between subpopulations separated by distances of approximately 2–3 km. Genome-wide association studies (GWAS) were performed to compare indoor/outdoor biting behavior and blood-seeking at dusk/dawn. A statistically significant association was observed between biting behavior and single nucleotide polymorphism (SNP) markers adjacent to the gene associated with cytochrome P450 (CYP) 4H14, which is associated with insecticide resistance. A statistically significant association between blood-seeking periodicity and SNP markers adjacent to genes associated with the circadian cycle was also observed. Conclusion: The data presented here suggest that low-coverage whole-genome sequencing with adequate processing is a powerful tool to genetically characterize vector populations at a microgeographic scale in malaria transmission areas, as well as for use in GWAS. Female mosquitoes entering houses to take a blood meal may be related to a specific CYP4H14 allele, and female timing of blood-seeking is related to circadian rhythm genes. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries Parasites and Vectors
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Nyssorhynchus darlingi en_US
dc.subject microgeographic dispersion en_US
dc.subject blood-seeking behavior en_US
dc.title Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s13071-022-05219-5
dc.relation.issn 1756-3305


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