dc.contributor.advisor |
Montes Delgado, Martín |
es_ES |
dc.contributor.advisor |
González Lagos, Elsa Violeta |
es_ES |
dc.contributor.author |
Gonzalez, Marcos Roberto |
es_ES |
dc.date.accessioned |
2022-06-09T20:58:28Z |
|
dc.date.available |
2022-06-09T20:58:28Z |
|
dc.date.issued |
2022 |
|
dc.identifier.other |
67504 |
es_ES |
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/11791 |
|
dc.description.abstract |
Antecedentes: El rol de los linfocitos T CD4 polifuncionales (Tpol) en tuberculosis latente de tuberculosis (LTBi) o HTLV-1 no está claramente definido. Objetivo: Describir la frecuencia de linfocitos Tpol en pacientes LTBi o HTLV-1. Métodos: Cinco pacientes con LTBi, 7 pacientes asintomáticos con HTLV-1 y 9 controles sanos fueron enrolados en este estudio. Células mononucleares de sangre periférica (PBMCs) de pacientes con LTBi fueron estimuladas con PPD. PBMCs de pacientes con HTLV-1 fueron estimuladas con PHA. Mediante citometría de flujo, se midió la frecuencia de Tpol productores de IFN-γ, TNF-α y/o IL-2. Resultados: Comparado con controles sanos, se observó una mayor frecuencia basal de linfocitos Tpol TNF-α+ IL-2+ en pacientes con HTLV-1 (0.0125% y 0.0006% respectivamente, p = 0.02). Tras estimulación con PPD, pacientes con LTBi presentaron mayor frecuencia de Tpol TNF-α+ IL-2+ (0.1086%) comparado con controles sanos (0.0292%) (p = 0.02). No se observó una diferencia significativa en la frecuencia total de linfocitos Tpol en pacientes HTLV-1 comparado con controles sanos (0.0217% y 0.0019% respectivamente, p = 0.06). Se encontró una diferencia significativa en la frecuencia total de linfocitos Tpol entre pacientes LTBi y controles sanos tras estimulación con PPD (0.3240% y 0.0810% respectivamente, p = 0.03). Conclusión: La mayor frecuencia de linfocitos Tpol en pacientes con LTBi indica un rol inflamatorio importante en la respuesta anti-tuberculosis, mediada principalmente por el subtipo TNF-α+ IL-2+. En pacientes con HTLV-1, la infección aumenta la frecuencia basal de Tpol sin afectar la capacidad de producción de citoquinas intracelulares. |
es_ES |
dc.description.abstract |
Background: The role of polyfunctional T CD4 lymphocytes (Tpol) in latent tuberculosis infection (LTBi) or HTLV-1 has not yet been elucidated. Objective: Determine the frequency of Tpol lymphocytes in patients with LTBi or HTLV-1. Methods: Five patients with LTBi, 7 asymptomatic patients with HTLV-1 and 9 healthy controls were enrolled in this study. Peripheral blood mononuclear cells (PBMCs) from LTBi patients were stimulated with PPD. PBMCs from HTLV-1 patients were stimulated with PHA. Frequencies of IFN-γ, TNF-α and/or IL-2-producing Tpol were assessed by flow cytometry. Results: There was a higher basal frequency of TNF-α+ IL-2+ Tpol in patients with HTLV-1 than in healthy controls (0.0125% and 0.0006% respectively, p = 0.02). There was a higher frequency of PPD-stimulated TNF-α+ IL-2+ Tpol in LTBi patients than in healthy controls (0.1086% and 0.0292% respectively, p = 0.02). There was no significant difference on overall Tpol frequency between HTLV-1 patients and healthy controls (0.0217% and 0.0019% respectively, p = 0.06). After PPD stimulation, overall Tpol frequency was significantly higher in LTBi patients than in healthy controls (0.3240% and 0.0810% respectively, p = 0.03). Conclusion: Higher Tpol frequency among LTBi patients suggests a key inflammatory role of this subset in anti-tuberculosis immune response, mediated mainly by the TNF-α+ IL-2+ subset. In patients with HTLV-1, viral infection induces a higher Tpol frequency without affecting production capacity of intracellular cytokines. |
es_ES |
dc.format |
application/pdf |
es_ES |
dc.language.iso |
spa |
es_ES |
dc.publisher |
Universidad Peruana Cayetano Heredia |
es_ES |
dc.rights |
info:eu-repo/semantics/openAccess |
es_ES |
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
es_ES |
dc.subject |
Linfocitos T Polifuncionales |
es_ES |
dc.subject |
Infección Latente de Tuberculosis |
es_ES |
dc.subject |
HTLV-1 |
es_ES |
dc.subject |
Citometría de Flujo |
es_ES |
dc.title |
Modulación inflamatoria de linfocitos T polifuncionales en tuberculosis y HTLV-1 |
es_ES |
dc.title.alternative |
Inflammatory modulation of polyfunctional T lymphocytes in tuberculosis and HTLV-1 |
es_ES |
dc.type |
info:eu-repo/semantics/bachelorThesis |
es_ES |
thesis.degree.name |
Médico Cirujano |
es_ES |
thesis.degree.grantor |
Universidad Peruana Cayetano Heredia. Facultad de Medicina Alberto Hurtado |
es_ES |
thesis.degree.discipline |
Medicina |
es_ES |
dc.publisher.country |
PE |
es_ES |
dc.subject.ocde |
http://purl.org/pe-repo/ocde/ford#3.02.06 |
es_ES |
dc.subject.ocde |
http://purl.org/pe-repo/ocde/ford#3.02.07 |
es_ES |
dc.subject.ocde |
http://purl.org/pe-repo/ocde/ford#3.03.08 |
es_ES |
renati.author.cext |
000750941 |
|
renati.advisor.orcid |
https://orcid.org/0000-0002-7427-347X |
es_ES |
renati.advisor.orcid |
https://orcid.org/0000-0002-8543-8188 |
es_ES |
renati.advisor.dni |
06663624 |
|
renati.advisor.dni |
09542294 |
|
renati.type |
http://purl.org/pe-repo/renati/type#trabajoDeInvestigacion |
es_ES |
renati.level |
http://purl.org/pe-repo/renati/nivel#tituloProfesional |
es_ES |
renati.discipline |
912016 |
es_ES |
renati.juror |
Mas Ubillús, Guiliana |
es_ES |
renati.juror |
Krapp López, Fiorella del Carmen |
es_ES |
renati.juror |
Otero Vegas, Larissa |
es_ES |