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Laboratory Evaluation of the DPP Syphilis Screen & Confirm Assay

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dc.contributor.author Vargas Rivera, Silver Keith
dc.contributor.author Qquellon Palacios, Luz Jazmín
dc.contributor.author Vasquez, F.
dc.contributor.author Konda, K. A.
dc.contributor.author Calvo, G.
dc.contributor.author Reyes-Diaz, M.
dc.contributor.author Caceres Palacios, Carlos Fernando
dc.contributor.author Klausner, J. D.
dc.date.accessioned 2022-06-25T20:36:44Z
dc.date.available 2022-06-25T20:36:44Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/11906
dc.description.abstract Because syphilis is a public health concern, new strategies and tools for detecting active syphilis cases should be evaluated for future implementation. We assessed the laboratory performance of the DPP Syphilis Screen & Confirm rapid immunodiagnostic test (Chembio Diagnostics, Medford, NY, USA), using visual reading and the manufacturer’s electronic test microreader, for detection of treponemal and nontreponemal antibodies in 383 fully characterized stored serum specimens. We used the Treponema pallidum particle agglutination (TPPA) test and rapid plasma reagin (RPR) test as reference tests for the DPP Syphilis Screen & Confirm assay treponemal and nontreponemal components, respectively. The sensitivity values for treponemal antibody detection by electronic reader and visual interpretation were 83.2% and 85.9%, respectively, with 100% specificity. For nontreponemal antibody detection, the sensitivity values were 65.7% and 69.0% and the specificity values were 88.7% and 89.4% for electronic reader and visual interpretation, respectively. There was excellent correlation between visual interpretation and the microreader for either component (kappa coefficient, 0.953). When restricting the analysis to RPR titers of ≥1:8, the sensitivity was 96.9% for either reading method; numerical microreader values showed good correlation with RPR titers (Spearman rho of 0.77). The DPP Syphilis Screen & Confirm assay showed good performance, compared to reference syphilis tests, using serum. Field evaluation studies should be done to validate its use for detection of active cases and for monitoring of treated syphilis patients. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Microbiology Spectrum
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject dual testing en_US
dc.subject point of care en_US
dc.subject immunoserology en_US
dc.subject syphilis en_US
dc.title Laboratory Evaluation of the DPP Syphilis Screen & Confirm Assay en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/spectrum.02642-21
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 2165-0497


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