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Porcine model of neurocysticercosis by intracarotid injection of Taenia solium oncospheres: Dose assessment, infection outcomes and serological responses

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dc.contributor.author Arroyo Hurtado, Gianfranco
dc.contributor.author Toribio, Luz
dc.contributor.author Vargas-Calla, A.
dc.contributor.author Calcina, J.F.
dc.contributor.author Bernal, Edson
dc.contributor.author Chile Andrade, Nancy
dc.contributor.author Zambrano, M.
dc.contributor.author Gomez-Puerta, L.A.
dc.contributor.author Chacaltana, J.
dc.contributor.author Marzal, Miguel
dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author O'Neal, Seth E.
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Cysticercosis Working Group in Peru
dc.date.accessioned 2022-08-15T20:11:09Z
dc.date.available 2022-08-15T20:11:09Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12029
dc.description.abstract BACKGROUND: Neurocysticercosis (NCC) is the infection of the human central nervous system (CNS) by Taenia solium larvae that cause significant neurological morbidity. Studies on NCC pathophysiology, host-parasite interactions or therapeutic agents are limited by the lack of suitable animal models. We have previously reported that carotid injection of activated T. solium oncospheres directs parasites into the CNS and consistently reproduces NCC. This study assessed the minimal dose required to consistently obtain NCC by intracarotid oncosphere injection and compared antigen and antibody response profiles by dose-group. METHODS/PRINCIPAL FINDINGS: Three groups of pigs were infected with either 2500 (n = 10), 5000 (n = 11), or 10000 (n = 10) oncospheres. Two pigs died during the study. Necropsy exam at day 150 post-infection (PI) demonstrated viable NCC in 21/29 pigs (72.4%), with higher NCC rates with increasing oncosphere doses (4/9 [44.4%], 9/11 [81.8%] and 8/9 [88.9%] for 2500, 5000, and 10000 oncospheres respectively, P for trend = 0.035). CNS cyst burden was also higher in pigs with increasing doses (P for trend = 0.008). Viable and degenerated muscle cysticerci were also found in all pigs, with degenerated cysticerci more frequent in the 2500 oncosphere dose-group. All pigs were positive for circulating parasite antigens on ELISA (Ag-ELISA) from day 14 PI; circulating antigens markedly increased at day 30 PI and remained high with plateau levels in pigs infected with either 5000 or 10000 oncospheres, but not in pigs infected with 2500 oncospheres. Specific antibodies appeared at day 30 PI and were not different between dose-groups. CONCLUSION/SIGNIFICANCE: Intracarotid injection of 5000 or more oncospheres produces high NCC rates in pigs with CNS cyst burdens like those usually found in human NCC, making this model appropriate for studies on the pathogenesis of NCC and the effects of antiparasitic treatment. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject animal en_US
dc.subject Animals en_US
dc.subject pig en_US
dc.subject Taenia solium en_US
dc.subject Swine en_US
dc.subject Neurocysticercosis en_US
dc.subject Cysticercus en_US
dc.subject neurocysticercosis en_US
dc.subject parasitology en_US
dc.subject swine disease en_US
dc.subject Swine Diseases en_US
dc.subject brain cyst en_US
dc.subject cysticercus (cestode larva) en_US
dc.subject Central Nervous System Cysts en_US
dc.title Porcine model of neurocysticercosis by intracarotid injection of Taenia solium oncospheres: Dose assessment, infection outcomes and serological responses en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0010449
dc.relation.issn 1935-2735


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