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Whole-Genome Characterisation of ESBL-Producing E. coli Isolated from Drinking Water and Dog Faeces from Rural Andean Households in Peru

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dc.contributor.author Medina-Pizzali, Maria Luisa
dc.contributor.author Venkatesh, A.
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Cuicapuza, Diego
dc.contributor.author Salmon-Mulanovich, Gabriela
dc.contributor.author Mäusezahl, Daniel
dc.contributor.author Hartinger Peña, Stella Maria
dc.date.accessioned 2022-08-15T20:11:09Z
dc.date.available 2022-08-15T20:11:09Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12041
dc.description.abstract E. coli that produce extended-spectrum β-lactamases (ESBLs) are major multidrug-resistant bacteria. In Peru, only a few reports have characterised the whole genome of ESBL enterobacteria. We aimed to confirm the identity and antimicrobial resistance (AMR) profile of two ESBL isolates from dog faeces and drinking water of rural Andean households and determine serotype, phylogroup, sequence type (ST)/clonal complex (CC), pathogenicity, virulence genes, ESBL genes, and their plasmids. To confirm the identity and AMR profiles, we used the VITEK®2 system. Whole-genome sequencing (WGS) and bioinformatics analysis were performed subsequently. Both isolates were identified as E. coli, with serotypes-:H46 and O9:H10, phylogroups E and A, and ST/CC 5259/-and 227/10, respectively. The isolates were ESBL-producing, carbapenem-resistant, and not harbouring carbapenemase-encoding genes. Isolate 1143 ST5259 harboured the astA gene, encoding the EAST1 heat-stable toxin. Both genomes carried ESBL genes (blaEC-15, blaCTX-M-8, and blaCTX-M-55 ). Nine plasmids were detected, namely IncR, IncFIC(FII), IncI, IncFIB(AP001918), Col(pHAD28), IncFII, IncFII(pHN7A8), IncI1, and IncFIB(AP001918). Finding these potentially pathogenic bacteria is worrisome given their sources and highlights the importance of One-Health research efforts in remote Andean communities. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Antibiotics
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject human en_US
dc.subject Peru en_US
dc.subject prevalence en_US
dc.subject nonhuman en_US
dc.subject animal experiment en_US
dc.subject antibiotic resistance en_US
dc.subject antibiotic sensitivity en_US
dc.subject cefotaxime en_US
dc.subject ceftazidime en_US
dc.subject cephalosporin en_US
dc.subject disk diffusion en_US
dc.subject ertapenem en_US
dc.subject Escherichia coli en_US
dc.subject extended spectrum beta lactamase en_US
dc.subject feces analysis en_US
dc.subject imipenem en_US
dc.subject meropenem en_US
dc.subject drinking water en_US
dc.subject rural area en_US
dc.subject one health en_US
dc.subject quality control en_US
dc.subject dog en_US
dc.subject RNA extraction en_US
dc.subject Western blotting en_US
dc.subject whole genome sequencing en_US
dc.subject piperacillin plus tazobactam en_US
dc.subject amikacin en_US
dc.subject whole-genome sequencing en_US
dc.subject ciprofloxacin en_US
dc.subject gentamicin en_US
dc.subject minimum inhibitory concentration en_US
dc.subject trimethoprim en_US
dc.subject microbiological examination en_US
dc.subject gene en_US
dc.subject fosfomycin en_US
dc.subject bioinformatics en_US
dc.subject cefepime en_US
dc.subject colistin en_US
dc.subject cefuroxime en_US
dc.subject quinolone en_US
dc.subject long untranslated RNA en_US
dc.subject cefazolin en_US
dc.subject genetic transfection en_US
dc.subject carbapenem en_US
dc.subject tigecycline en_US
dc.subject uvomorulin en_US
dc.subject sultamicillin en_US
dc.subject cell invasion en_US
dc.subject bla shv gene en_US
dc.subject bla tem gene en_US
dc.subject blactx m 10 gene en_US
dc.subject blactx m 2 en_US
dc.subject blactx m 3 gene en_US
dc.subject blaoxa gene en_US
dc.subject carbapenem resistance en_US
dc.subject ESBL-producing E. coli en_US
dc.subject phylogenomic analysis en_US
dc.subject transwell assay en_US
dc.subject vimentin en_US
dc.title Whole-Genome Characterisation of ESBL-Producing E. coli Isolated from Drinking Water and Dog Faeces from Rural Andean Households in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/antibiotics11050692
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.05
dc.relation.issn 2079-6382


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