Universidad Peruana Cayetano Heredia
Whole-Genome Characterisation of ESBL-Producing E. coli Isolated from Drinking Water and Dog Faeces from Rural Andean Households in Peru
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| dc.contributor.author | Medina-Pizzali, Maria Luisa | |
| dc.contributor.author | Venkatesh, A. | |
| dc.contributor.author | Riveros Ramirez, Maribel Denise | |
| dc.contributor.author | Cuicapuza, Diego | |
| dc.contributor.author | Salmon-Mulanovich, Gabriela | |
| dc.contributor.author | Mäusezahl, Daniel | |
| dc.contributor.author | Hartinger Peña, Stella Maria | |
| dc.date.accessioned | 2022-08-15T20:11:09Z | |
| dc.date.available | 2022-08-15T20:11:09Z | |
| dc.date.issued | 2022 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/12041 | |
| dc.description.abstract | E. coli that produce extended-spectrum β-lactamases (ESBLs) are major multidrug-resistant bacteria. In Peru, only a few reports have characterised the whole genome of ESBL enterobacteria. We aimed to confirm the identity and antimicrobial resistance (AMR) profile of two ESBL isolates from dog faeces and drinking water of rural Andean households and determine serotype, phylogroup, sequence type (ST)/clonal complex (CC), pathogenicity, virulence genes, ESBL genes, and their plasmids. To confirm the identity and AMR profiles, we used the VITEK®2 system. Whole-genome sequencing (WGS) and bioinformatics analysis were performed subsequently. Both isolates were identified as E. coli, with serotypes-:H46 and O9:H10, phylogroups E and A, and ST/CC 5259/-and 227/10, respectively. The isolates were ESBL-producing, carbapenem-resistant, and not harbouring carbapenemase-encoding genes. Isolate 1143 ST5259 harboured the astA gene, encoding the EAST1 heat-stable toxin. Both genomes carried ESBL genes (blaEC-15, blaCTX-M-8, and blaCTX-M-55 ). Nine plasmids were detected, namely IncR, IncFIC(FII), IncI, IncFIB(AP001918), Col(pHAD28), IncFII, IncFII(pHN7A8), IncI1, and IncFIB(AP001918). Finding these potentially pathogenic bacteria is worrisome given their sources and highlights the importance of One-Health research efforts in remote Andean communities. | en_US |
| dc.language.iso | eng | |
| dc.publisher | MDPI | |
| dc.relation.ispartofseries | Antibiotics | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | Article | en_US |
| dc.subject | controlled study | en_US |
| dc.subject | human | en_US |
| dc.subject | Peru | en_US |
| dc.subject | prevalence | en_US |
| dc.subject | nonhuman | en_US |
| dc.subject | animal experiment | en_US |
| dc.subject | antibiotic resistance | en_US |
| dc.subject | antibiotic sensitivity | en_US |
| dc.subject | cefotaxime | en_US |
| dc.subject | ceftazidime | en_US |
| dc.subject | cephalosporin | en_US |
| dc.subject | disk diffusion | en_US |
| dc.subject | ertapenem | en_US |
| dc.subject | Escherichia coli | en_US |
| dc.subject | extended spectrum beta lactamase | en_US |
| dc.subject | feces analysis | en_US |
| dc.subject | imipenem | en_US |
| dc.subject | meropenem | en_US |
| dc.subject | drinking water | en_US |
| dc.subject | rural area | en_US |
| dc.subject | one health | en_US |
| dc.subject | quality control | en_US |
| dc.subject | dog | en_US |
| dc.subject | RNA extraction | en_US |
| dc.subject | Western blotting | en_US |
| dc.subject | whole genome sequencing | en_US |
| dc.subject | piperacillin plus tazobactam | en_US |
| dc.subject | amikacin | en_US |
| dc.subject | whole-genome sequencing | en_US |
| dc.subject | ciprofloxacin | en_US |
| dc.subject | gentamicin | en_US |
| dc.subject | minimum inhibitory concentration | en_US |
| dc.subject | trimethoprim | en_US |
| dc.subject | microbiological examination | en_US |
| dc.subject | gene | en_US |
| dc.subject | fosfomycin | en_US |
| dc.subject | bioinformatics | en_US |
| dc.subject | cefepime | en_US |
| dc.subject | colistin | en_US |
| dc.subject | cefuroxime | en_US |
| dc.subject | quinolone | en_US |
| dc.subject | long untranslated RNA | en_US |
| dc.subject | cefazolin | en_US |
| dc.subject | genetic transfection | en_US |
| dc.subject | carbapenem | en_US |
| dc.subject | tigecycline | en_US |
| dc.subject | uvomorulin | en_US |
| dc.subject | sultamicillin | en_US |
| dc.subject | cell invasion | en_US |
| dc.subject | bla shv gene | en_US |
| dc.subject | bla tem gene | en_US |
| dc.subject | blactx m 10 gene | en_US |
| dc.subject | blactx m 2 | en_US |
| dc.subject | blactx m 3 gene | en_US |
| dc.subject | blaoxa gene | en_US |
| dc.subject | carbapenem resistance | en_US |
| dc.subject | ESBL-producing E. coli | en_US |
| dc.subject | phylogenomic analysis | en_US |
| dc.subject | transwell assay | en_US |
| dc.subject | vimentin | en_US |
| dc.title | Whole-Genome Characterisation of ESBL-Producing E. coli Isolated from Drinking Water and Dog Faeces from Rural Andean Households in Peru | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.3390/antibiotics11050692 | |
| dc.relation.issn | 2079-6382 |
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