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The Differences in the Susceptibility Patterns to Triclabendazole Sulfoxide in Field Isolates of Fasciola hepatica Are Associated with Geographic, Seasonal, and Morphometric Variations

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dc.contributor.author Fernandez-Baca, Martha V.
dc.contributor.author Hoban, C.
dc.contributor.author Ore, Rodrigo A.
dc.contributor.author Ortiz, P.
dc.contributor.author Choi, Y.-J.
dc.contributor.author Murga-Moreno, C.
dc.contributor.author Mitreva, M.
dc.contributor.author Cabada, Miguel M.
dc.date.accessioned 2022-08-15T20:11:11Z
dc.date.available 2022-08-15T20:11:11Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12071
dc.description.abstract Triclabendazole (TCBZ) resistance is an emerging problem in fascioliasis that is not well understood. Studies including small numbers of parasites fail to capture the complexity of susceptibility variations between and within Fasciola hepatica populations. As the first step to studying the complex resistant phenotype–genotype associations, we characterized a large sample of adult F. hepatica with diverging TCBZ susceptibility. We collected parasites from naturally infected livestock slaughtered in the Cusco and Cajamarca regions of Peru. These parasites were exposed to TCBZ sulfoxide (TCBZ.SO) in vitro to determine their susceptibility. We used a motility score to determine the parasite’s viability. We titrated drug concentrations and times to detect 20% non-viable (susceptible conditions) or 80% non-viable (resistant conditions) parasites. We exposed 3348 fully motile parasites to susceptible (n = 1565) or resistant (n = 1783) conditions. Three hundred and forty-one (21.8%) were classified as susceptible and 462 (25.9%) were classified as resistant. More resistant parasites were found in Cusco than in Cajamarca (p < 0.001). Resistant parasites varied by slaughterhouse (p < 0.001), month of the year (p = 0.008), fluke length (p = 0.016), and year of collection (p < 0.001). The in vitro susceptibility to TCBZ.SO in wildtype F. hepatica was associated with geography, season, and morphometry. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Pathogens
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject Peru en_US
dc.subject nonhuman en_US
dc.subject animal experiment en_US
dc.subject antibiotic sensitivity en_US
dc.subject seasonal variation en_US
dc.subject geography en_US
dc.subject slaughterhouse en_US
dc.subject resistance en_US
dc.subject triclabendazole en_US
dc.subject fascioliasis en_US
dc.subject Fasciola hepatica en_US
dc.subject parasite en_US
dc.subject morphometry en_US
dc.subject natural infection en_US
dc.subject genotype phenotype correlation en_US
dc.subject parasite viability en_US
dc.subject trematode en_US
dc.subject body movement en_US
dc.subject cajamarca en_US
dc.subject cusco en_US
dc.subject sulfoxide en_US
dc.title The Differences in the Susceptibility Patterns to Triclabendazole Sulfoxide in Field Isolates of Fasciola hepatica Are Associated with Geographic, Seasonal, and Morphometric Variations en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/pathogens11060625
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05
dc.relation.issn 2076-0817


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