dc.contributor.author |
Millones Gomez, Pablo Alejandro |
|
dc.contributor.author |
Requena Mendizabal, Margarita Fe |
|
dc.contributor.author |
Calla Poma, Roger Damaso |
|
dc.contributor.author |
Rosales Cifuentes, Tania Valentina |
|
dc.contributor.author |
Malpartida Quispe, Federico Martin |
|
dc.contributor.author |
Maurtua Torres, Dora Jesus |
|
dc.contributor.author |
Bacilio Amaranto, Reyma Evelyn |
|
dc.contributor.author |
Minchon Medina, Carlos Alberto |
|
dc.contributor.author |
Ponce Contreras, Lusin Antonio |
|
dc.date.accessioned |
2022-09-09T18:52:22Z |
|
dc.date.available |
2022-09-09T18:52:22Z |
|
dc.date.issued |
2022 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/12144 |
|
dc.description.abstract |
Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii (p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Association of Support to Oral Health Research |
|
dc.relation.ispartofseries |
Pesquisa Brasileira em Odontopediatria e Clinica Integrada |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Psidium |
en_US |
dc.subject |
Biofilms |
en_US |
dc.subject |
Gram-Negative Bacteria |
en_US |
dc.title |
Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1590/pboci.2022.028 |
|
dc.relation.issn |
1983-4632 |
|