DSpace Repository

Diagnosis of Leishmania Using the Polymerase Chain Reaction: a Simplified Procedure for Field Work

Show simple item record

dc.contributor.author Lopez, Martín
dc.contributor.author Inga, R.
dc.contributor.author Cangalaya, M.
dc.contributor.author Echevarria Zarate, Juan Ignacio
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Orrego, C.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.date.accessioned 2022-10-09T17:42:33Z
dc.date.available 2022-10-09T17:42:33Z
dc.date.issued 1993
dc.identifier.uri https://hdl.handle.net/20.500.12866/12283
dc.description.abstract Oligonucleotide primers directed to the minicircle kinetoplast DNA of Leishmania strains supported enzymatic amplification of this DNA by the polymerase chain reaction (PCR). A single product of 70 basepairs was obtained from parasites belonging exclusively to the L. braziliensis complex. Direct sequencing of the amplified product confirmed its minicircle origin. Skin biopsy specimens from human patients were used directly for the PCR. A pulse incubation of such specimens with deoxyribonuclease I prior to the PCR increased the reliability of the assay. Nuclease disruption of the kinetoplast network was expected to make more copies of the minicircle accessible to amplification. Comparative results between the PCR and conventional parasitologic detection procedures indicate that the DNA detection approach presented is by far more sensitive for diagnostic purposes. Innovations in the PCR protocol are presented that adapt the diagnosis of leishmaniasis to settings with minimal equipment and that are distant from central laboratories. en_US
dc.language.iso eng
dc.publisher American Journal of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Polymerase Chain Reaction en_US
dc.subject Base Sequence en_US
dc.subject DNA, Circular/analysis en_US
dc.subject DNA, Kinetoplast en_US
dc.subject DNA, Protozoan/analysis en_US
dc.subject Evaluation Studies as Topic en_US
dc.subject Leishmania braziliensis/genetics en_US
dc.subject Leishmaniasis, Cutaneous/diagnosis en_US
dc.subject Leishmaniasis, Mucocutaneous/diagnosis en_US
dc.subject Molecular Sequence Data en_US
dc.subject Oligodeoxyribonucleotides/chemistry en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Skin/parasitology en_US
dc.title Diagnosis of Leishmania Using the Polymerase Chain Reaction: a Simplified Procedure for Field Work en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.1993.49.348
dc.relation.issn 1476-1645


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics