Universidad Peruana Cayetano Heredia

The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia

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dc.contributor.author Victoir, K.
dc.contributor.author Bañuls, A.L.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Hamers, R.
dc.contributor.author Noël, S.
dc.contributor.author De Doncker, S.
dc.contributor.author Le Ray, D.
dc.contributor.author Tibayrenc, M.
dc.contributor.author Dujardin, J.C.
dc.date.accessioned 2022-10-09T22:16:52Z
dc.date.available 2022-10-09T22:16:52Z
dc.date.issued 1998
dc.identifier.uri https://hdl.handle.net/20.500.12866/12299
dc.description.abstract In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63-RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR-RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a reference. On the one hand gp63-RFLP evidenced an extensive polymorphism and revealed specific markers for subgenus, species and geographical populations: congruence with MLEE was demonstrated statistically. The particular interest of gp63-RFLP was illustrated by infra- specific polymorphism, because of the possible relationship with phenotype diversity. On the other hand intra-genic amplification was less resolutive than gp63-RFLP, but also allowed discrimination of the 2 subgenera (PCR alone) and all the species tested in the subgenus Viannia (PCR-RFLP). PCR-RFLP presents an important operational advantage as it allows genetic characterization of minute amounts of parasites, using Leishmania specific primers. The polymorphism revealed by gp63-RFLP and PCR-RFLP illustrates the very high genomic and genetic plasticity of gp63 genes. en_US
dc.language.iso eng
dc.publisher Cambridge University Press
dc.relation.ispartofseries Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Gene organization en_US
dc.subject Gp63 en_US
dc.subject Leishmania en_US
dc.subject PCR en_US
dc.subject Taxonomy en_US
dc.subject Viannia subgenus en_US
dc.title The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1017/s0031182098002789
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1469-8161


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