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Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis

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dc.contributor.author Montoya, Y.
dc.contributor.author Leon, C.
dc.contributor.author Talledo Albujar, Michael John
dc.contributor.author Nolasco, O.
dc.contributor.author Padilla, C.
dc.contributor.author Muñoz-Najar, U.
dc.contributor.author Barker, D.C.
dc.date.accessioned 2022-10-09T22:16:52Z
dc.date.available 2022-10-09T22:16:52Z
dc.date.issued 1997
dc.identifier.uri https://hdl.handle.net/20.500.12866/12302
dc.description.abstract The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4:) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Transactions of the Royal Society of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Diagnosis en_US
dc.subject Leishmania peruviana en_US
dc.subject Leishmaniasis en_US
dc.subject Recombinant proteins en_US
dc.title Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/S0035-9203(97)90520-4
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1878-3503


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