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Fasciola hepatica cysteine proteinases: Immunodominant antigens in human fascioliasis

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dc.contributor.author Cordova, M.
dc.contributor.author Herrera Velit, Rosa Patricia
dc.contributor.author Nopo, L.
dc.contributor.author Bellatin, J.
dc.contributor.author Naquira Velarde, Cesar Gabriel
dc.contributor.author Guerra, H.
dc.contributor.author Espinoza, J.R.
dc.date.accessioned 2022-10-09T22:16:52Z
dc.date.available 2022-10-09T22:16:52Z
dc.date.issued 1997
dc.identifier.uri https://hdl.handle.net/20.500.12866/12303
dc.description.abstract Fasciola hepatica adult worm cysteine proteinases were active-site, affinity radio-labeled with benzyloxicarbonyl-L-tyrosine-L-alanine diazomethylketone (Z-Tyr125I-Ala-CHN2). Sera from patients with fascioliasis and from rabbits experimentally infected with F. hepatica immunoprecipitated the radiolabeled parasite cysteine proteinases in immunoelectrophoresis assays. Two purified antigens were identified as part of the complex mosaic of antigens immunoprecipitated by the sera of infected patients. These antigens (Fas1 and Fas2) have been shown to be an important part of the Fharc2 precipitin band used for serologic diagnosis in humans and cattle. They showed cysteine proteinase activity with different proteolytic specificities and partial identity in double immunodiffusion assays. The results obtained in this work show that the Fas1 and Fas2 antigens are sensitive and specific antigens for diagnosis of this serious helminthic disease in humans and other susceptible hosts. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Fasciola Hepatica en_US
dc.subject Fascioliasis en_US
dc.subject Animals en_US
dc.title Fasciola hepatica cysteine proteinases: Immunodominant antigens in human fascioliasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.1997.57.660
dc.relation.issn 1476-1645


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