Immunodiagnosis of human cysticercosis (Taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (ELISA), an antigen-ELISA, and an enzyme-linked immunoelectrotransfer blot (EITB) assay in Peru. The Cysticercosis Working Group in Peru (CWG)

García Lescano, Héctor Hugo; Gilman, Robert Hugh; Pilcher, J; Torres, P; Diaz, J; Verastegui Pimentel, Manuela Renee; Montenegro, T; Miranda, E; Tsang, V; Gallo López-Aliaga, Carla Maria

dc.contributor.author	Diaz, J
dc.contributor.author	Verastegui Pimentel, Manuela Renee
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Tsang, V
dc.contributor.author	Pilcher, J
dc.contributor.author	Gallo López-Aliaga, Carla Maria
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Torres, P
dc.contributor.author	Montenegro, T
dc.contributor.author	Miranda, E
dc.date.accessioned	2022-10-10T03:26:12Z
dc.date.available	2022-10-10T03:26:12Z
dc.date.issued	1992
dc.identifier.uri	https://hdl.handle.net/20.500.12866/12349
dc.description.abstract	We compared results of an enzyme-linked immunosorbent assay (ELISA) and an enzyme-linked immunoelectrotransfer blot (EITB) assay for the diagnosis of cysticercosis in sera and cerebrospinal fluid (CSF). Sera from 34 patients with confirmed cysticercosis were tested by both ELISA and EITB assays. Cerebrospinal fluid from some of these patients was also tested by ELISA for the presence of antibody (AB-ELISA) (n = 21) and antigen (AG-ELISA) (n = 15). Specificity in sera was examined by testing 51 serum samples from Bangladesh, where cysticercosis is not endemic. Cross-reactivity was evaluated in sera from patients with Echinococcus granulosus (hydatid) and Hymenolepis nana infections. Sensitivity in detecting cysticercosis in sera was 94% by EITB and 65% by AB-ELISA (P less than 0.01). Sensitivities in the CSF tested by EITB, AB-ELISA, and AG-ELISA were 86%, 62%, and 67%, respectively. The specificity of the EITB was 100%, while that of AB-ELISA was 63% (P less than 0.01). Cross-reactions occurred in the AB-ELISA with 11% and 20% of sera from hydatid and H. nana patients, respectively. Our results demonstrate that the EITB is the best assay available for the diagnosis of cysticercosis in both sera and CSF.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Cysticercosis	en_US
dc.subject	ELISA	en_US
dc.subject	EITB	en_US
dc.title	Immunodiagnosis of human cysticercosis (Taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (ELISA), an antigen-ELISA, and an enzyme-linked immunoelectrotransfer blot (EITB) assay in Peru. The Cysticercosis Working Group in Peru (CWG)	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.1992.46.610
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645