Universidad Peruana Cayetano Heredia

Immunoinformatic analysis of the whole proteome for vaccine design: An application to Clostridium perfringens

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dc.contributor.author Soto, L.F.
dc.contributor.author Romaní, A.C.
dc.contributor.author Jiménez-Avalos, Gabriel
dc.contributor.author Silva, Y.
dc.contributor.author Ordinola-Ramirez, C.M.
dc.contributor.author Lopez Lapa, R.M.
dc.contributor.author Requena, D.
dc.date.accessioned 2022-10-12T18:25:58Z
dc.date.available 2022-10-12T18:25:58Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12377
dc.description.abstract Clostridium perfringens is a dangerous bacterium and known biological warfare weapon associated with several diseases, whose lethal toxins can produce necrosis in humans. However, there is no safe and fully effective vaccine against C. perfringens for humans yet. To address this problem, we computationally screened its whole proteome, identifying highly immunogenic proteins, domains, and epitopes. First, we identified that the proteins with the highest epitope density are Collagenase A, Exo-alpha-sialidase, alpha n-acetylglucosaminidase and hyaluronoglucosaminidase, representing potential recombinant vaccine candidates. Second, we further explored the toxins, finding that the non-toxic domain of Perfringolysin O is enriched in CTL and HTL epitopes. This domain could be used as a potential sub-unit vaccine to combat gas gangrene. And third, we designed a multi-epitope protein containing 24 HTL-epitopes and 34 CTL-epitopes from extracellular regions of transmembrane proteins. Also, we analyzed the structural properties of this novel protein using molecular dynamics. Altogether, we are presenting a thorough immunoinformatic exploration of the whole proteome of C. perfringens, as well as promising whole-protein, domain-based and multi-epitope vaccine candidates. These can be evaluated in preclinical trials to assess their immunogenicity and protection against C. perfringens infection. en_US
dc.language.iso eng
dc.publisher Frontiers Media
dc.relation.ispartofseries Frontiers in Immunology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject immunoinformatics en_US
dc.subject Clostridium perfringens en_US
dc.subject epitope en_US
dc.subject toxin en_US
dc.subject vaccine en_US
dc.subject molecular dynamics en_US
dc.title Immunoinformatic analysis of the whole proteome for vaccine design: An application to Clostridium perfringens en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3389/fimmu.2022.942907
dc.relation.issn 1664-3224


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