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Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis

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dc.contributor.author Arana, Marlyn
dc.contributor.author Evans, D. A.
dc.contributor.author Zolessi, A.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.date.accessioned 2022-10-25T19:54:09Z
dc.date.available 2022-10-25T19:54:09Z
dc.date.issued 1990
dc.identifier.uri https://hdl.handle.net/20.500.12866/12451
dc.description.abstract Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Transactions of the Royal Society of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Electrophoresis en_US
dc.subject Cellulose Acetate en_US
dc.subject Isoelectric Focusing en_US
dc.subject Leishmaniasis Mucocutaneous parasitology en_US
dc.subject Malate Dehydrogenase analysis en_US
dc.subject Mannose-6-Phosphate Isomerase analysis en_US
dc.title Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/0035-9203(90)90025-a
dc.relation.issn 1878-3503


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