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Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen

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dc.contributor.author Huaman, Maria Cecilia.
dc.contributor.author Sato, Y.
dc.contributor.author Aguilar Olano, José Luis
dc.contributor.author Terashima, A.
dc.contributor.author Guerra, Humberto
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Kanbara, H.
dc.date.accessioned 2022-10-25T19:54:10Z
dc.date.available 2022-10-25T19:54:10Z
dc.date.issued 2003
dc.identifier.uri https://hdl.handle.net/20.500.12866/12461
dc.description.abstract Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Transactions of the Royal Society of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject strongyloidiasisllStrongyloides venezuelensislldiagnosisllgelatin particle indirect agglutination testllenzyme-linked immunosorbent assayllPeru en_US
dc.title Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/S0035-9203(03)80017-2
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1878-3503


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