Universidad Peruana Cayetano Heredia

Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg yolk Agar in a miniwell format

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dc.contributor.author Vasquez, A.
dc.contributor.author Valdez, Y.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author McDonald, J. J.
dc.contributor.author Westblom, T. U.
dc.contributor.author Berg, D.
dc.contributor.author Mayta, H.
dc.contributor.author Gutierrez, V.
dc.contributor.author Berendson, R.
dc.contributor.author León-Barúa, Raúl
dc.contributor.author Ramirez-Ramos, A.
dc.contributor.author Recavarren-Arce, S.
dc.contributor.author Watanabe, J.
dc.date.accessioned 2022-10-25T19:54:10Z
dc.date.available 2022-10-25T19:54:10Z
dc.date.issued 1996
dc.identifier.uri https://hdl.handle.net/20.500.12866/12470
dc.description.abstract Resistance of Helicobacter pylori to metronidazole often causes failure of commonly used combination drug treatment regimens. We determined the MICs of metronidazole and clarithromycin against 18 H. pylori strains from Peru using tetrazolium egg yolk (TEY) agar. The MIC results obtained by agar dilution with petri dishes were compared with the results found through a miniwell format. The results of the two protocols for measuring drug susceptibility differed by no more than 1 dilution in all cases. On TEY agar, bright-red H. pylori colonies were easy to identify against a yellow background. Sixty-one percent (11 of 18) of the strains were resistant to metronidazole (MIC, > or = 4 micrograms/ml) and 50% (9 of 18) were resistant to clarithromycin (MIC, > or = 0.125 micrograms/ml), whereas none (0 of 5) of the strains tested were resistant to tetracycline (MIC, > or = 1 micrograms/ml). Thus, the prevalence of metronidazole and clarithromycin resistance in Peru is higher than that in developed regions of the world. The miniwell plate with TEY agar allows easy H. pylori colony identification, requires about one-third less of the costly medium necessary for petri dish assaying, conserves space, and yields MICs equivalent to those with agar dilution in petri dishes. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Helicobacter pylori en_US
dc.subject metronidazole en_US
dc.subject Peru en_US
dc.title Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg yolk Agar in a miniwell format en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.34.5.1232-1234.1996
dc.relation.issn 1098-660X


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