Universidad Peruana Cayetano Heredia

"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru.

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dc.contributor.author Garcia Bardales, Paul F.
dc.contributor.author Schiaffino Salazar, Francesca
dc.contributor.author Huynh, Steven
dc.contributor.author Paredes Olortegui, Maribel
dc.contributor.author Penataro Yori, Pablo
dc.contributor.author Pinedo Vasquez, Tackeshy
dc.contributor.author Manzanares Villanueva, Katia
dc.contributor.author Curico Huansi, Greisi E.
dc.contributor.author Shapiama Lopez, Wagner V.
dc.contributor.author Cooper, Kerry K.
dc.contributor.author Parker, Craig T.
dc.contributor.author Kosek, Margaret N.
dc.date.accessioned 2022-11-15T23:04:41Z
dc.date.available 2022-11-15T23:04:41Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12594
dc.description.abstract A working hypothesis is that less common species of Campylobacter (other than C. jejuni and C. coli) play a role in enteric disease among children in low resource settings and explain the gap between the detection of Campylobacter using culture and culture independent methods. "Candidatus Campylobacter infans" (C. infans), was recently detected in stool samples from children and hypothesized to play a role in Campylobacter epidemiology in low- and middle-income countries (LMIC). This study determined the prevalence of C. infans in symptomatic and asymptomatic stool samples from children living in Iquitos, Peru. Stool samples from 215 children with diarrhea and 50 stool samples from children without diarrhea under the age of two were evaluated using a multiplex qPCR assay to detect Campylobacter spp. (16S rRNA), Campylobacter jejuni / Campylobacter coli (cadF gene), C. infans (lpxA), and Shigella spp. (ipaH). C. infans was detected in 7.9% (17/215) symptomatic samples and 4.0% (2/50) asymptomatic samples. The association between diarrhea and the presence of these targets was evaluated using univariate logistic regressions. C. infans was not associated with diarrhea. Fifty-one percent (75/146) of Campylobacter positive fecal samples were negative for C. jejuni, C. coli, and C. infans via qPCR. Shotgun metagenomics confirmed the presence of C. infans among 13 out of 14 positive C. infans positive stool samples. C infans explained only 20.7% of the diagnostic gap in stools from children with diarrhea and 16.7% of the gap in children without diarrhea. We posit that poor cadF primer performance better explains the observed gap than the prevalence of atypical non-C. jejuni/coli species. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Campylobacter en_US
dc.subject Diarrhea en_US
dc.subject Metagenomics en_US
dc.subject Campylobacter jejuni en_US
dc.subject Shotgun sequencing en_US
dc.subject Ribosomal RNA en_US
dc.subject Shigellosis en_US
dc.subject Saliva en_US
dc.title "Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0010869
dc.relation.issn 1935-2735


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