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A Smartphone-Based Low-Cost Inverted Laser Fluorescence Microscope for Disease Diagnosis

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dc.contributor.author Ormachea, O.
dc.contributor.author Villazón, A.
dc.contributor.author Rodriguez, P.
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2022-12-14T14:25:33Z
dc.date.available 2022-12-14T14:25:33Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12869
dc.description.abstract Fluorescence microscopy is an important tool for disease diagnosis, often requiring costly optical components, such as fluorescence filter cubes and high-power light sources. Due to its high cost, conventional fluorescence microscopy cannot be fully exploited in low-income settings. Smartphone-based fluorescence microscopy becomes an interesting low-cost alternative, but raises challenges in the optical system. We present the development of a low-cost inverted laser fluorescence microscope that uses a smartphone to visualize the fluorescence image of biological samples. Our fluorescence microscope uses a laser-based simplified optical filter system that provides analog optical filtering capabilities of a fluorescence filter cube. Firstly, we validated our inverted optical filtering by visualizing microbeads labeled with three different fluorescent compounds or fluorophores commonly used for disease diagnosis. Secondly, we validated the disease diagnosis capabilities by comparing the results of our device with those of a commercial fluorescence microscope. We successfully detected and visualized Trypanosoma cruzi parasites, responsible for the Chagas infectious disease and the presence of Antineutrophil cytoplasmic antibodies of the ANCA non-communicable autoimmune disease. The samples were labeled with the fluorescein isothiocyanate (FITC) fluorophore, one of the most commonly used fluorophores for disease diagnosis. Our device provides a 400× magnification and is at least one order of magnitude cheaper than conventional commercial fluorescence microscopes. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Biosensors
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject low-cost fluorescence microscopy en_US
dc.subject smartphone microscopy en_US
dc.subject 3D-printed devices en_US
dc.subject laser-based microscopy en_US
dc.title A Smartphone-Based Low-Cost Inverted Laser Fluorescence Microscope for Disease Diagnosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/bios12110960
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.04.04
dc.relation.issn 1873-4219


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info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

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