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Production of endoglin-specific heavy chain antibody fragments (VHHs) microarrays for whole-cell SPR imaging

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dc.contributor.author Quintana, M.
dc.contributor.author Espinoza, José R.
dc.contributor.author Roupioz, Y.
dc.date.accessioned 2022-12-14T14:25:36Z
dc.date.available 2022-12-14T14:25:36Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12911
dc.description.abstract Cell-based Surface Plasmon Resonance imaging (SPRi) with anti-endoglin VHH antibodies microarray on a gold-covered biochip was used to characterize the binding to endoglin expressed on the surface membrane of SC cells. SPRi showed the specific capture of SC cells by the immobilized anti-endoglin VHHs, but not of THP-1 cells that do not express endoglin on their plasmatic membrane. The SPRi reflectivity shifts with SC cells bound by anti-endoglin VHHs ranged from 3.59 to 5.54% (average 4.66 ± 1.18%) and with THP-1 cells from 2.23 to 3.05% (average 2.62 ± 1.32% (p < 0.001)) and the SC cell densities with anti-endoglin VHHs ranged from 417 to 747.7, (average 548 cells/2.28 × 105 μm2 ± 132). Anti-endoglin VHHs bind epitopes located in the extracellular domains of endoglin with different avidity as suggested by the differences in SC cell densities. The cell-based SPRi of anti-endoglin VHH microarrays can be used both in studies aimed at a better understanding of endoglin function in living cells and in VHH applications as nanoprobes. en_US
dc.description.sponsorship Este trabajo fue financiado por FONDECYT [número de contrato 111-2015]. es_PE
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Sensors and Actuators Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antibodies en_US
dc.subject Cytology en_US
dc.subject Biochip en_US
dc.subject Biochips en_US
dc.subject Cell density en_US
dc.subject Cell-based en_US
dc.subject Cells en_US
dc.subject Endoglin en_US
dc.subject Heavy-chain antibody fragments en_US
dc.subject Microarray en_US
dc.subject SC cell en_US
dc.subject SC cells en_US
dc.subject SPR imaging en_US
dc.subject SPRi en_US
dc.subject Surface plasmon resonance en_US
dc.subject Surface plasmon resonance imaging en_US
dc.subject THP-1 cells en_US
dc.subject VHH en_US
dc.subject Whole cell en_US
dc.title Production of endoglin-specific heavy chain antibody fragments (VHHs) microarrays for whole-cell SPR imaging en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.snr.2022.100131
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 2666-0539


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