Universidad Peruana Cayetano Heredia
Production of endoglin-specific heavy chain antibody fragments (VHHs) microarrays for whole-cell SPR imaging
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| dc.contributor.author | Quintana, M. | |
| dc.contributor.author | Espinoza, José R. | |
| dc.contributor.author | Roupioz, Y. | |
| dc.date.accessioned | 2022-12-14T14:25:36Z | |
| dc.date.available | 2022-12-14T14:25:36Z | |
| dc.date.issued | 2022 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/12911 | |
| dc.description.abstract | Cell-based Surface Plasmon Resonance imaging (SPRi) with anti-endoglin VHH antibodies microarray on a gold-covered biochip was used to characterize the binding to endoglin expressed on the surface membrane of SC cells. SPRi showed the specific capture of SC cells by the immobilized anti-endoglin VHHs, but not of THP-1 cells that do not express endoglin on their plasmatic membrane. The SPRi reflectivity shifts with SC cells bound by anti-endoglin VHHs ranged from 3.59 to 5.54% (average 4.66 ± 1.18%) and with THP-1 cells from 2.23 to 3.05% (average 2.62 ± 1.32% (p < 0.001)) and the SC cell densities with anti-endoglin VHHs ranged from 417 to 747.7, (average 548 cells/2.28 × 105 μm2 ± 132). Anti-endoglin VHHs bind epitopes located in the extracellular domains of endoglin with different avidity as suggested by the differences in SC cell densities. The cell-based SPRi of anti-endoglin VHH microarrays can be used both in studies aimed at a better understanding of endoglin function in living cells and in VHH applications as nanoprobes. | en_US |
| dc.description.sponsorship | Este trabajo fue financiado por FONDECYT [número de contrato 111-2015]. | es_PE |
| dc.language.iso | eng | |
| dc.publisher | Elsevier | |
| dc.relation.ispartofseries | Sensors and Actuators Reports | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | Antibodies | en_US |
| dc.subject | Cytology | en_US |
| dc.subject | Biochip | en_US |
| dc.subject | Biochips | en_US |
| dc.subject | Cell density | en_US |
| dc.subject | Cell-based | en_US |
| dc.subject | Cells | en_US |
| dc.subject | Endoglin | en_US |
| dc.subject | Heavy-chain antibody fragments | en_US |
| dc.subject | Microarray | en_US |
| dc.subject | SC cell | en_US |
| dc.subject | SC cells | en_US |
| dc.subject | SPR imaging | en_US |
| dc.subject | SPRi | en_US |
| dc.subject | Surface plasmon resonance | en_US |
| dc.subject | Surface plasmon resonance imaging | en_US |
| dc.subject | THP-1 cells | en_US |
| dc.subject | VHH | en_US |
| dc.subject | Whole cell | en_US |
| dc.title | Production of endoglin-specific heavy chain antibody fragments (VHHs) microarrays for whole-cell SPR imaging | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.1016/j.snr.2022.100131 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.01 | |
| dc.relation.issn | 2666-0539 |
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