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Evaluation of three alternatives cost-effective culture media for Mycobacterium tuberculosis detection and drug susceptibility determination using the microscopic observation drug susceptibility (MODS) assay

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dc.contributor.author Rodriguez, Jhojailith
dc.contributor.author Alcántara, R.
dc.contributor.author Rodríguez, Joseline
dc.contributor.author Vargas, J.
dc.contributor.author Roncal, Elisa
dc.contributor.author Antiparra, Ricardo
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Grandjean, Louis
dc.contributor.author Moore, David Alexander James
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Sheen Cortavarria, Patricia
dc.date.accessioned 2022-12-14T14:25:36Z
dc.date.available 2022-12-14T14:25:36Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12919
dc.description.abstract Tuberculosis phenotypic detection assays are commonly used in low-resource countries. Therefore, reliable detection methods are crucial for early diagnosis and treatment. The microscopic observation drug susceptibility (MODS) assay is a culture-based test to detect Mycobacterium tuberculosis and characterize drug resistance in 7–10 days directly from sputum. The use of MODS is limited by the availability of supplies necessary for preparing the enriched culture. In this study, we evaluated three dry culture media that are easier to produce and cheaper than the standard one used in MODS [1]: an unsterilized powder-based mixed (Boldú et al., 2007) [2], a sterile-lyophilized medium, and (Sengstake et al., 2017) [3] an irradiated powder-based mixed. Mycobacterial growth and drug susceptibility were evaluated for rifampin, isoniazid, and pyrazinamide (PZA). The alternative cultures were evaluated using 282 sputum samples with positive acid-fast smears. No significant differences were observed in the positivity test rates. The positivity time showed high correlations (Rho) of 0.925, 0.889, and 0.866 between each of the three alternative media and the standard. Susceptibility testing for MDR and PZA showed an excellent concordance of 1 compared to the reference test. These results demonstrate that dry culture media are appropriate and advantageous for use in MODS in low-resource settings. en_US
dc.description.sponsorship Este trabajo fue financiado por PROCIENCIA/CONCYTEC [número de contrato 157-2018]. es_PE
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Tuberculosis
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject culture media en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject drug susceptibility en_US
dc.subject microscopic observation drug susceptibility en_US
dc.subject MODS en_US
dc.title Evaluation of three alternatives cost-effective culture media for Mycobacterium tuberculosis detection and drug susceptibility determination using the microscopic observation drug susceptibility (MODS) assay en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.tube.2022.102273
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.07
dc.relation.issn 1873-281X


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