Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age from Peru.

Riveros Ramirez, Maribel Denise; Durand Vara, David Percy; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim; Pons, Maria J.

dc.contributor.author	Riveros Ramirez, Maribel Denise
dc.contributor.author	Pons, Maria J.
dc.contributor.author	Durand Vara, David Percy
dc.contributor.author	Ochoa Woodell, Theresa Jean
dc.contributor.author	Ruiz, Joaquim
dc.coverage.spatial	Lima, Perú
dc.date.accessioned	2023-02-20T13:28:10Z
dc.date.available	2023-02-20T13:28:10Z
dc.date.issued	2023
dc.identifier.uri	https://hdl.handle.net/20.500.12866/13150
dc.description.abstract	Class 1 and Class 2 integrons are mobilizable elements able to carry a variety of antibiotic resistance determinants. In the present study, Class 1 and 2 integrons present in 355 pathogenic Escherichia coli (285 diarrheagenic, of these 129 were enteropathogenic, 90 enteroaggregative, 66 enterotoxigenic, and 70 bacteremic) isolated from healthy and ill children under age 5 from periurban areas of Lima, Peru, were characterized. The presence of integrase 1 and 2 was established by polymerase chain reaction (PCR), and variable regions were grouped by PCR-restriction fragment length polymorphism and subsequent sequencing. Antimicrobial resistance was established by disk diffusion. Ninety-seven isolates (27.3%) presented integrase 1, and 16 (4.5%) presented integrase 2 (P < 0.0001); in addition, seven (2.0%) isolates, six diarrheagenic and one bacteremic, presented both integrase genes. The presence of integrase 1 was more frequent among bacteremic isolates (P = 0.0004). Variable regions were amplified in 76/120 (63.3%) isolates with up to 14 gene arrangements. The most prevalent gene cassettes were those encoding dihydrofolate reductases as well as aminoglycoside modifying enzymes. Of note, Class 1 integrons tended to be associated with the presence of extended-spectrum beta-lactamases (ESBLs). A variety of Class 1 and 2 integrons were detected in diarrheagenic and bacteremic E. coli, demonstrating the heterogeneity of variable regions circulating in the area. The association of integrons with ESBLs is worrisome and has an impact on the development of multidrug resistance.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Integrons	en_US
dc.subject	Escherichia coli	en_US
dc.subject	Strains	en_US
dc.subject	Diarrhea	en_US
dc.subject	Bacteremia	en_US
dc.subject	Children	en_US
dc.subject	Peru	en_US
dc.subject.mesh	Integrons
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Sprains and Strains
dc.subject.mesh	Diarrhea
dc.subject.mesh	Bacteremia
dc.subject.mesh	Child
dc.subject.mesh	Peru
dc.title	Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age from Peru.	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.22-0239
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645