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Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age from Peru.

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dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Pons, Maria J.
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ruiz, Joaquim
dc.coverage.spatial Lima, Perú
dc.date.accessioned 2023-02-20T13:28:10Z
dc.date.available 2023-02-20T13:28:10Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13150
dc.description.abstract Class 1 and Class 2 integrons are mobilizable elements able to carry a variety of antibiotic resistance determinants. In the present study, Class 1 and 2 integrons present in 355 pathogenic Escherichia coli (285 diarrheagenic, of these 129 were enteropathogenic, 90 enteroaggregative, 66 enterotoxigenic, and 70 bacteremic) isolated from healthy and ill children under age 5 from periurban areas of Lima, Peru, were characterized. The presence of integrase 1 and 2 was established by polymerase chain reaction (PCR), and variable regions were grouped by PCR-restriction fragment length polymorphism and subsequent sequencing. Antimicrobial resistance was established by disk diffusion. Ninety-seven isolates (27.3%) presented integrase 1, and 16 (4.5%) presented integrase 2 (P < 0.0001); in addition, seven (2.0%) isolates, six diarrheagenic and one bacteremic, presented both integrase genes. The presence of integrase 1 was more frequent among bacteremic isolates (P = 0.0004). Variable regions were amplified in 76/120 (63.3%) isolates with up to 14 gene arrangements. The most prevalent gene cassettes were those encoding dihydrofolate reductases as well as aminoglycoside modifying enzymes. Of note, Class 1 integrons tended to be associated with the presence of extended-spectrum beta-lactamases (ESBLs). A variety of Class 1 and 2 integrons were detected in diarrheagenic and bacteremic E. coli, demonstrating the heterogeneity of variable regions circulating in the area. The association of integrons with ESBLs is worrisome and has an impact on the development of multidrug resistance. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Integrons en_US
dc.subject Escherichia coli en_US
dc.subject Strains en_US
dc.subject Diarrhea en_US
dc.subject Bacteremia en_US
dc.subject Children en_US
dc.subject Peru en_US
dc.subject.mesh Integrons
dc.subject.mesh Escherichia coli
dc.subject.mesh Sprains and Strains
dc.subject.mesh Diarrhea
dc.subject.mesh Bacteremia
dc.subject.mesh Child
dc.subject.mesh Peru
dc.title Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age from Peru. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.22-0239
dc.relation.issn 1476-1645


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