Universidad Peruana Cayetano Heredia
Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru
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| dc.contributor.author | Rosales-Rimache, Jaime | |
| dc.contributor.author | Nunayalle-Vargas, Magda | |
| dc.contributor.author | Rueda Torres, Lenin Vladimir | |
| dc.contributor.author | Inolopú Cucche, Jorge Luis | |
| dc.coverage.spatial | Centro Materno Infantil Ollantay | |
| dc.date.accessioned | 2023-02-20T13:28:11Z | |
| dc.date.available | 2023-02-20T13:28:11Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/13163 | |
| dc.description.abstract | Background: Sputum smear microscopy (SSM) is a screening test used to diagnose tuberculosis (TB); however, its performance and sensitivity are relatively low, which can lead to false negatives. We designed a cross-sectional study to estimate the performance of SSM that includes a pretreatment based on sputum digestion with bleach (sodium hypochlorite) for the diagnosis of TB. Methods: We evaluated 73 sputum samples from patients with a diagnosis of TB confirmed by the Xpert MTB/RIF test and 114 samples from patients without TB. We performed sputum digestion using a 5% sodium hypochlorite solution, centrifuged at 2000 rpm for 15 min. We prepared smears for direct and bleach-treated SSM and used Ziehl-Neelsen staining. Results: The bleach-treated SSM obtained absolute identification of the cases of TB confirmed by the Xpert test, compared to 95.9% identified by the direct smear method (without bleach treatment). We also found a significant increase (p < 0.001) in the recovery of acid-fast bacilli (AFB) obtained by the bleach-treated SSM (293.8 +/- 215.1 AFB) compared to the direct SSM method (222.9 +/- 195.5 AFB). The AUC of the bleach-treated SSM and direct SSM was 100% and 96.6%, respectively. Conclusion: The bleach-treated SSM performs better than the direct SSM in identifying AFB and increasing the bacillary count in the sputum samples. | en_US |
| dc.language.iso | eng | |
| dc.publisher | MDPI | |
| dc.relation.ispartofseries | International Journal of Environmental Research and Public Health | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | tuberculosis | en_US |
| dc.subject | bacilloscopy | en_US |
| dc.subject | sputum | en_US |
| dc.subject.mesh | Tuberculosis | |
| dc.subject.mesh | Sputum | |
| dc.title | Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.3390/ijerph20010135 | |
| dc.relation.issn | 1660-4601 |
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