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Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru

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dc.contributor.author Rosales-Rimache, Jaime
dc.contributor.author Nunayalle-Vargas, Magda
dc.contributor.author Rueda Torres, Lenin Vladimir
dc.contributor.author Inolopú Cucche, Jorge Luis
dc.coverage.spatial Centro Materno Infantil Ollantay
dc.date.accessioned 2023-02-20T13:28:11Z
dc.date.available 2023-02-20T13:28:11Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13163
dc.description.abstract Background: Sputum smear microscopy (SSM) is a screening test used to diagnose tuberculosis (TB); however, its performance and sensitivity are relatively low, which can lead to false negatives. We designed a cross-sectional study to estimate the performance of SSM that includes a pretreatment based on sputum digestion with bleach (sodium hypochlorite) for the diagnosis of TB. Methods: We evaluated 73 sputum samples from patients with a diagnosis of TB confirmed by the Xpert MTB/RIF test and 114 samples from patients without TB. We performed sputum digestion using a 5% sodium hypochlorite solution, centrifuged at 2000 rpm for 15 min. We prepared smears for direct and bleach-treated SSM and used Ziehl-Neelsen staining. Results: The bleach-treated SSM obtained absolute identification of the cases of TB confirmed by the Xpert test, compared to 95.9% identified by the direct smear method (without bleach treatment). We also found a significant increase (p < 0.001) in the recovery of acid-fast bacilli (AFB) obtained by the bleach-treated SSM (293.8 +/- 215.1 AFB) compared to the direct SSM method (222.9 +/- 195.5 AFB). The AUC of the bleach-treated SSM and direct SSM was 100% and 96.6%, respectively. Conclusion: The bleach-treated SSM performs better than the direct SSM in identifying AFB and increasing the bacillary count in the sputum samples. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries International Journal of Environmental Research and Public Health
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject tuberculosis en_US
dc.subject bacilloscopy en_US
dc.subject sputum en_US
dc.subject.mesh Tuberculosis
dc.subject.mesh Sputum
dc.title Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/ijerph20010135
dc.relation.issn 1660-4601

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