Universidad Peruana Cayetano Heredia

A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis

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dc.contributor.author Juscamayta López, Julio Eduardo
dc.contributor.author Valdivia, F.
dc.contributor.author Soto, M.P.
dc.contributor.author Nureña, B.
dc.contributor.author Horna, H.
dc.coverage.spatial Instituto Nacional de Salud, Lima, Perú
dc.date.accessioned 2023-04-16T04:38:11Z
dc.date.available 2023-04-16T04:38:11Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13349
dc.description.abstract Despite widespread vaccination, Bordetella pertussis continues to cause pertussis infections worldwide, leaving infants at the highest risk of severe illness and death, while people around them are likely the main sources of infection and rapidly spread the disease. Rapid and less complex molecular testing for the specific and timely diagnosis of pertussis remains a challenge that could help to prevent the disease from worsening and prevent its transmission. We aimed to develop and validate a colorimetric loop-mediated isothermal amplification (LAMP) assay using a new target uvrD_2 informed by the pangenome for the specific and early detection of B. pertussis. Compared to that of multitarget quantitative polymerase chain reaction (multitarget qPCR) using a large clinical DNA specimen (n = 600), the diagnostic sensitivity and specificity of the uvrD_2 LAMP assay were 100.0% and 98.6%, respectively, with a 99.7% degree of agreement between the two assays. The novel colorimetric uvrD_2 LAMP assay is highly sensitive and specific for detecting B. pertussis DNA in nasopharyngeal swabs and showed similar diagnostic accuracy to complex and high-cost multitarget qPCR, but it is faster, simpler, and inexpensive, which makes it very helpful for the reliable and timely diagnosis of pertussis in primary health care and resource-limited settings. © 2023, The Author(s). en_US
dc.description.sponsorship Este trabajo fue financiado por el Instituto Nacional de Salud del Perú en el marco del proyecto de investigación “Desarrollo y evaluación de un método rápido basado en LAMP para el diagnóstico molecular y vigilancia de la infección por Bordetella pertussis ” [número de subvención RD N°374-2019 -OGITT/INS]. es_PE
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartofseries Scientific Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Molecular Diagnostic Techniques en_US
dc.subject Nucleic Acid Amplification Techniques en_US
dc.subject *Bordetella pertussis/genetics en_US
dc.subject *Whooping Cough/diagnosis en_US
dc.title A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41598-023-29773-9
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 2045-2322


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