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Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru.

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dc.contributor.author Kattenberg, Johanna Helena
dc.contributor.author Van Dijk, Norbert J.
dc.contributor.author Fernandez Miñope, Carlos Alberto
dc.contributor.author Guetens, Pieter
dc.contributor.author Mutsaers, Mathijs
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Rosanas-Urgell, Anna
dc.coverage.spatial Perú
dc.date.accessioned 2023-04-16T04:38:15Z
dc.date.available 2023-04-16T04:38:15Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13391
dc.description.abstract Malaria molecular surveillance has great potential to support national malaria control programs (NMCPs), informing policy for its control and elimination. Here, we present a new three-day workflow for targeted resequencing of markers in 13 resistance-associated genes, histidine rich protein 2 and 3 (hrp2&3), a country (Peru)-specific 28 SNP-barcode for population genetic analysis, and apical membrane antigen 1 (ama1), using Illumina short-read sequencing technology. The assay applies a multiplex PCR approach to amplify all genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once, therefore having great potential for implementation into routine surveillance practice by NMCPs. The assay can be performed on routinely collected filter paper blood spots and can be easily adapted to different regions to investigate either regional trends or in-country epidemiological changes. en_US
dc.language.iso eng
dc.publisher Bio-Protocol
dc.relation.ispartofseries Bio-protocol
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Plasmodium falciparum en_US
dc.subject AmpliSeq custom assay en_US
dc.subject Drug resistance en_US
dc.subject Malaria en_US
dc.subject Molecular surveillance en_US
dc.subject Sequencing en_US
dc.title Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.21769/BioProtoc.4621
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 2331-8325


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