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Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays.

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dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Toribio Salazar, Luz Milagros
dc.contributor.author Guzmán Dueñas, Carolina del Rosario
dc.contributor.author Arroyo Hurtado, Gianfranco
dc.contributor.author Espinoza Guerrero, Cindy
dc.contributor.author Saavedra, Herbert
dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author Dorny, Pierre
dc.contributor.author O'Neal, Seth E.
dc.contributor.author García Lescano, Héctor Hugo
dc.coverage.spatial Instituto Nacional de Ciencias Neurologicas, Lima, Peru
dc.date.accessioned 2023-05-19T14:08:34Z
dc.date.available 2023-05-19T14:08:34Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13528
dc.description.abstract Monoclonal antibody (mAb)-based enzyme-linked immunosorbent assay (ELISA) is a complementary diagnosis technique for neurocysticercosis (NCC), which detects circulating parasite antigen (Ag) indicative of viable infection and Ag levels that correlate well with the parasite burden. In this study, we compared the performance of two Ag-ELISA techniques for the detection of NCC. We assessed the agreement between our in-house TsW8/TsW5 Ag-ELISA and the widely used B158/B60 Ag-ELISA for measuring T. solium antigen levels in the sera from 113 patients with calcified, parenchymal, and subarachnoid NCC. Concordance was demonstrated evaluating the limits of agreement (LoAs) stratified by the type of NCC. Both ELISA's detected 47/48 (97.8%) subarachnoid NCC cases. In parenchymal and calcified NCC, the B158/B60 Ag-ELISA detected 19/24 (79.2%) and 18/41 (43.9%) cases, while the TsW8/TsW5 Ag-ELISA detected 21/24 (87.5%) and 13/41 (31.7%), respectively. Parenchymal and calcified NCC obtained a perfect agreement (100%), indicating that all sample results were within the predicted LoA, while for subarachnoid NCC, the agreement was 89.6%. The high concordance between the assays was confirmed by Lin's concordance coefficient (LCC = 0.97). Patients with viable parenchymal NCC (LCC = 0.95) obtained the highest concordance between assays, followed by subarachnoid NCC (LCC = 0.93) and calcified NCC (LCC = 0.92). The TsW8/TsW5 Ag-ELISA and B158/B60 Ag-ELISA showed high Ag measurement correlations across diverse types of NCC. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Pathogens
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject neurocysticercosis en_US
dc.subject Taenia solium en_US
dc.subject Ag-ELISA en_US
dc.subject monoclonal antibodies en_US
dc.subject agreement en_US
dc.subject.mesh Neurocisticercosis
dc.subject.mesh Taenia solium
dc.subject.mesh Anticuerpos Monoclonales
dc.subject.mesh Contrato de Transferencia
dc.title Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/pathogens12040566
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05
dc.relation.issn 2076-0817


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