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Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts

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dc.contributor.author Jaramillo-Cañas, Winston
dc.contributor.author Britto Bisso, Frank
dc.contributor.author Fernandez Valiente, Cesar Antonio
dc.contributor.author Casado, Fanny L.
dc.date.accessioned 2023-06-12T16:25:56Z
dc.date.available 2023-06-12T16:25:56Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13634
dc.description.abstract Demineralized bone matrix (DBM) is considered one of the most reliable bone tissue grafts for regular surgical use, as it provides a scaffold that is structurally like native bone, and that enhances bone regeneration. However, commercially available DBM products are not suited for surgical restitutions of large bones. Therefore, each Tissue Bank is urged to implement their own demineralization protocol, which usually does not meet the high demand for bone grafting. In this project, we developed an open source system for medium-scale manufacturing of DBM grafts from human cadaveric donors to automate the demineralization protocol and improve its reproducibility. The device consists in (1) unidirectional flow reaction chamber, where the demineralization protocol takes place; (2) automated syringe pump, which controls the reagent́s inlet and vacuum; and (3) reagent dispenser, for the management of the reagents need for the demineralization protocol. Validation of the device included histological analysis, DNA quantification temperature regulation, electrochemiluminescence and colorimetric protocols, followed by the optimization of physicochemical parameters based on Response Surface Methodology. The results showed values of residual lipids and calcium within standardized ranges, and the maintenance of the structural integrity of the DBM, demonstrating the capacity of the system to support the proposed demineralization protocol. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries HardwareX
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Perfusion system en_US
dc.subject Bone grafting en_US
dc.subject Tissue Engineering en_US
dc.subject.mesh Perfusión
dc.subject.mesh Trasplante Óseo
dc.subject.mesh Ingeniería de Tejidos
dc.title Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ohx.2022.e00378
dc.relation.issn 2468-0672


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