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Development and Laboratory Evaluation of a Simple, Field-Applicable Coproantigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Taeniasis in Northern Peru.

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dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Wardle, Melissa T.
dc.contributor.author Gamboa Morán, Ricardo
dc.contributor.author Elizalde Pizarro, Mayra Yajayra
dc.contributor.author Vilchez Barreto, Percy Mcquen
dc.contributor.author Rodriguez, Silvia
dc.contributor.author Gilman, Robert H.
dc.contributor.author Gonzalez, Armando E.
dc.contributor.author O'Neal, Seth E.
dc.contributor.author García Lescano, Héctor Hugo
dc.coverage.spatial Norte del Perú
dc.date.accessioned 2023-07-18T16:18:55Z
dc.date.available 2023-07-18T16:18:55Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/13918
dc.description.abstract Coproantigen detection by enzyme-linked immunosorbent assay (coAg ELISA) is a vital tool for detecting and treating cases of Taenia solium taeniasis. However, the assay's procedures require costly materials and sophisticated equipment, which are typically inaccessible in rural settings where the disease is endemic. To overcome these barriers, we developed and evaluated a field-applicable coAg ELISA. The field coAg ELISA was developed and evaluated across four phases using known positive and negative stool samples collected from northern Peru. Phase I focused on field assay development, phase II on a small-scale performance evaluation, phase III on a large-scale evaluation, and phase IV on the use and reliability of a colorimetric scale card. All samples were processed using the field and standard assay procedures and compared using signal-to-noise ratios, correlation tests, performance characteristics, and agreement statistics where appropriate. The field coAg ELISA using reagents stored at -20°C and commercially available water and milk powder, and relying on spontaneous separation of the supernatant, had performance comparable to the standard assay. The field coAg ELISA was strongly correlated with the standard in both the small- and large-scale laboratory evaluation (r = 0.99 and r = 0.98, respectively). Finally, the field assay had an almost perfect agreement between independent readers (kappa = 0.975) and between each reader and the spectrophotometer. The field coAg ELISA demonstrated performance comparable to the standard, providing a low-cost alternative to the standard assay for identifying cases of intestinal taeniasis in a low-resource setting. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Enzyme-linked immunosorbent assay en_US
dc.subject Cysticercosis en_US
dc.subject Diagnosis en_US
dc.subject Taenia solium en_US
dc.subject Taeniasis en_US
dc.subject Neurocysticercosis en_US
dc.subject.mesh Ensayo de Inmunoadsorción Enzimática
dc.subject.mesh Cisticercosis
dc.subject.mesh Diagnóstico
dc.subject.mesh Taenia solium
dc.subject.mesh Teniasis
dc.subject.mesh Neurocisticercosis
dc.title Development and Laboratory Evaluation of a Simple, Field-Applicable Coproantigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Taeniasis in Northern Peru. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/jcm.00282-23
dc.relation.issn 1098-660X


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