No impact of helminth coinfection in patients with smear positive tuberculosis on immunoglobulin levels using a novel method measuring Mycobacterium  tuberculosis-specific antibodies.

Blomgran, Robert; Stendahl, Olle; Gilman, Robert Hugh; Schön, Thomas; Pushpamithran, Giggil; Segelmark, Mårten; Méndez Aranda, Melissa Marlene; Skoglund, Camilla; Olsson, Fanny

dc.contributor.author	Pushpamithran, Giggil
dc.contributor.author	Skoglund, Camilla
dc.contributor.author	Olsson, Fanny
dc.contributor.author	Méndez Aranda, Melissa Marlene
dc.contributor.author	Schön, Thomas
dc.contributor.author	Segelmark, Mårten
dc.contributor.author	Stendahl, Olle
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Blomgran, Robert
dc.coverage.spatial	Iquitos, Loreto, Perú
dc.coverage.spatial	Lima, Perú
dc.coverage.spatial	Suecia
dc.date.accessioned	2023-07-18T16:18:56Z
dc.date.available	2023-07-18T16:18:56Z
dc.date.issued	2023
dc.identifier.uri	https://hdl.handle.net/20.500.12866/13922
dc.description.abstract	Helminth/tuberculosis (TB)-coinfection can reduce cell-mediated immunity against Mycobacterium tuberculosis (Mtb) and increase disease severity, although the effects are highly helminth species dependent. Mtb have long been ranked as the number one single infectious agent claiming the most lives. The only licensed vaccine for TB (BCG) offers highly variable protection against TB, and almost no protection against transmission of Mtb. In recent few years the identification of naturally occurring antibodies in humans that are protective during Mtb infection has reignited the interest in adaptive humoral immunity against TB and its possible implementation in novel TB vaccine design. The effects of helminth/TB coinfection on the humoral response against Mtb during active pulmonary TB are however still unclear, and specifically the effect by globally prevalent helminth species such as Ascaris lumbricoides, Strongyloides stercoralis, Ancylostoma duodenale, Trichuris trichiura. Plasma samples from smear positive TB patients were used to measure both total and Mtb-specific antibody responses in a Peruvian endemic setting where these helminths are dominating. Mtb-specific antibodies were detected by a novel approach coating ELISA-plates with a Mtb cell-membrane fraction (CDC1551) that contains a broad range of Mtb surface proteins. Compared to controls without helminths or TB, helminth/TB coinfected patients had high levels of Mtb-specific IgG (including an IgG1 and IgG2 subclass response) and IgM, which were similarly increased in TB patients without helminth infection. These data, indicate that helminth/TB coinfected have a sustained humoral response against Mtb at the level of active TB only. More studies on the species-specific impact of helminths on the adaptive humoral response against Mtb using a larger sample size, and in relation to TB disease severity, are needed.	en_US
dc.language.iso	eng
dc.publisher	Springer
dc.relation.ispartofseries	Allergy, Asthma, & Clinical Immunology
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Mycobacterium tuberculosis	en_US
dc.subject	Active pulmonary TB	en_US
dc.subject	Helminth coinfection	en_US
dc.subject	Adaptive humoral immune response	en_US
dc.subject	Mtb-specific IgG IgA IgM	en_US
dc.subject.mesh	Mycobacterium tuberculosis
dc.subject.mesh	Tuberculosis Pulmonar
dc.subject.mesh	Helmintos
dc.subject.mesh	Inmunidad Humoral
dc.subject.mesh	Inmunoglobulina G
dc.subject.mesh	Inmunoglobulina A
dc.subject.mesh	Inmunoglobulina M
dc.title	No impact of helminth coinfection in patients with smear positive tuberculosis on immunoglobulin levels using a novel method measuring Mycobacterium tuberculosis-specific antibodies.	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1186/s13223-023-00808-0
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.02.16
dc.relation.issn	1710-1492