dc.contributor.author |
Malaga, Jose L. |
|
dc.contributor.author |
Pajuelo Travezaño, Monica Jhenny |
|
dc.contributor.author |
Okamoto, Michiko |
|
dc.contributor.author |
Tsinda, Emmanuel Kagning |
|
dc.contributor.author |
Otani, Kanako |
|
dc.contributor.author |
Tsukayama Cisneros, Pablo |
|
dc.contributor.author |
Mascaro Rivera, Lucero Nancy |
|
dc.contributor.author |
Cuicapuza Artega, Diego Bernhard |
|
dc.contributor.author |
Katsumi, Masamichi |
|
dc.contributor.author |
Kawamura, Kazuhisa |
|
dc.contributor.author |
Nishimura, Hidekazu |
|
dc.contributor.author |
Sakagami, Akie |
|
dc.contributor.author |
Ueki, Yo |
|
dc.contributor.author |
Omiya, Suguru |
|
dc.contributor.author |
Okamoto, Satoshi |
|
dc.contributor.author |
Nakayama, Asami |
|
dc.contributor.author |
Fujimaki, Shin-Ichi |
|
dc.contributor.author |
Yu, Chuyao |
|
dc.contributor.author |
Azam, Sikandar |
|
dc.contributor.author |
Kodama, Eiichi |
|
dc.contributor.author |
Dapat, Clyde |
|
dc.contributor.author |
Oshitani, Hitoshi |
|
dc.contributor.author |
Saito, Mayuko |
|
dc.date.accessioned |
2023-07-18T16:18:56Z |
|
dc.date.available |
2023-07-18T16:18:56Z |
|
dc.date.issued |
2023 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/13929 |
|
dc.description.abstract |
Rapid molecular testing for severe acute respiratory coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase amplification using a lateral flow assay (RT-RPA-LF) allows rapid RNA detection without thermal cyclers. In this study, we developed two assays to detect SARS-CoV-2 nucleocapsid (N) gene and Omicron BA.1 spike (S) gene-specific deletion-insertion mutations (del211/ins214). Both tests had a detection limit of 10 copies/µL in vitro and the detection time was approximately 35 min from incubation to detection. The sensitivities of SARS-CoV-2 (N) RT-RPA-LF by viral load categories were 100% for clinical samples with high (>9015.7 copies/µL, cycle quantification (Cq): < 25) and moderate (385.5-9015.7 copies/µL, Cq: 25-29.9) viral load, 83.3% for low (16.5-385.5 copies/µL, Cq: 30-34.9), and 14.3% for very low (<16.5 copies/µL, Cq: 35-40). The sensitivities of the Omicron BA.1 (S) RT-RPA-LF were 94.9%, 78%, 23.8%, and 0%, respectively, and the specificity against non-BA.1 SARS-CoV-2-positive samples was 96%. The assays seemed more sensitive than rapid antigen detection in moderate viral load samples. Although implementation in resource-limited settings requires additional improvements, deletion-insertion mutations were successfully detected by the RT-RPA-LF technique. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
MDPI |
|
dc.relation.ispartofseries |
Viruses |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
SARS-CoV-2 |
en_US |
dc.subject |
Variant of concern (VOC) |
en_US |
dc.subject |
Deletion–insertion mutation |
en_US |
dc.subject |
COVID-19 |
en_US |
dc.subject |
Recombinase polymerase amplification (RPA) |
en_US |
dc.subject.mesh |
SARS-CoV-2 |
|
dc.subject.mesh |
Mutación |
|
dc.subject.mesh |
COVID-19 |
|
dc.subject.mesh |
Reacción en Cadena de la Polimerasa |
|
dc.title |
Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion-Insertion Mutation in S-Protein Gene. |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.3390/v15061254 |
|
dc.relation.issn |
1999-4915 |
|