Universidad Peruana Cayetano Heredia
Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion-Insertion Mutation in S-Protein Gene.
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| dc.contributor.author | Malaga, Jose L. | |
| dc.contributor.author | Pajuelo Travezaño, Monica Jhenny | |
| dc.contributor.author | Okamoto, Michiko | |
| dc.contributor.author | Tsinda, Emmanuel Kagning | |
| dc.contributor.author | Otani, Kanako | |
| dc.contributor.author | Tsukayama Cisneros, Pablo | |
| dc.contributor.author | Mascaro Rivera, Lucero Nancy | |
| dc.contributor.author | Cuicapuza Artega, Diego Bernhard | |
| dc.contributor.author | Katsumi, Masamichi | |
| dc.contributor.author | Kawamura, Kazuhisa | |
| dc.contributor.author | Nishimura, Hidekazu | |
| dc.contributor.author | Sakagami, Akie | |
| dc.contributor.author | Ueki, Yo | |
| dc.contributor.author | Omiya, Suguru | |
| dc.contributor.author | Okamoto, Satoshi | |
| dc.contributor.author | Nakayama, Asami | |
| dc.contributor.author | Fujimaki, Shin-Ichi | |
| dc.contributor.author | Yu, Chuyao | |
| dc.contributor.author | Azam, Sikandar | |
| dc.contributor.author | Kodama, Eiichi | |
| dc.contributor.author | Dapat, Clyde | |
| dc.contributor.author | Oshitani, Hitoshi | |
| dc.contributor.author | Saito, Mayuko | |
| dc.date.accessioned | 2023-07-18T16:18:56Z | |
| dc.date.available | 2023-07-18T16:18:56Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/13929 | |
| dc.description.abstract | Rapid molecular testing for severe acute respiratory coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase amplification using a lateral flow assay (RT-RPA-LF) allows rapid RNA detection without thermal cyclers. In this study, we developed two assays to detect SARS-CoV-2 nucleocapsid (N) gene and Omicron BA.1 spike (S) gene-specific deletion-insertion mutations (del211/ins214). Both tests had a detection limit of 10 copies/µL in vitro and the detection time was approximately 35 min from incubation to detection. The sensitivities of SARS-CoV-2 (N) RT-RPA-LF by viral load categories were 100% for clinical samples with high (>9015.7 copies/µL, cycle quantification (Cq): < 25) and moderate (385.5-9015.7 copies/µL, Cq: 25-29.9) viral load, 83.3% for low (16.5-385.5 copies/µL, Cq: 30-34.9), and 14.3% for very low (<16.5 copies/µL, Cq: 35-40). The sensitivities of the Omicron BA.1 (S) RT-RPA-LF were 94.9%, 78%, 23.8%, and 0%, respectively, and the specificity against non-BA.1 SARS-CoV-2-positive samples was 96%. The assays seemed more sensitive than rapid antigen detection in moderate viral load samples. Although implementation in resource-limited settings requires additional improvements, deletion-insertion mutations were successfully detected by the RT-RPA-LF technique. | en_US |
| dc.language.iso | eng | |
| dc.publisher | MDPI | |
| dc.relation.ispartofseries | Viruses | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | SARS-CoV-2 | en_US |
| dc.subject | Variant of concern (VOC) | en_US |
| dc.subject | Deletion–insertion mutation | en_US |
| dc.subject | COVID-19 | en_US |
| dc.subject | Recombinase polymerase amplification (RPA) | en_US |
| dc.subject.mesh | SARS-CoV-2 | |
| dc.subject.mesh | Mutación | |
| dc.subject.mesh | COVID-19 | |
| dc.subject.mesh | Reacción en Cadena de la Polimerasa | |
| dc.title | Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion-Insertion Mutation in S-Protein Gene. | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.3390/v15061254 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.02 | |
| dc.relation.issn | 1999-4915 |
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