Universidad Peruana Cayetano Heredia

Identification and evaluation in-vitro of conserved peptides with high affinity to MHC-I as potential protective epitopes for Newcastle disease virus vaccines

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dc.contributor.author Tataje-Lavanda, L.
dc.contributor.author Málaga Machaca, Edith Silvia
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Mayta Huatuco, E.
dc.contributor.author Icochea, E.
dc.contributor.author Fernández-Díaz, M.
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2023-12-05T17:48:01Z
dc.date.available 2023-12-05T17:48:01Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/14637
dc.description.abstract Background: Newcastle disease (ND) is a major threat to the poultry industry, leading to significant economic losses. The current ND vaccines, usually based on active or attenuated strains, are only partially effective and can cause adverse effects post-vaccination. Therefore, the development of safer and more efficient vaccines is necessary. Epitopes represent the antigenic portion of the pathogen and their identification and use for immunization could lead to safer and more effective vaccines. However, the prediction of protective epitopes for a pathogen is a major challenge, especially taking into account the immune system of the target species. Results: In this study, we utilized an artificial intelligence algorithm to predict ND virus (NDV) peptides that exhibit high affinity to the chicken MHC-I complex. We selected the peptides that are conserved across different NDV genotypes and absent in the chicken proteome. From the filtered peptides, we synthesized the five peptides with the highest affinities for the L, HN, and F proteins of NDV. We evaluated these peptides in-vitro for their ability to elicit cell-mediated immunity, which was measured by the lymphocyte proliferation in spleen cells of chickens previously immunized with NDV. Conclusions: Our study identified five peptides with high affinity to MHC-I that have the potential to serve as protective epitopes and could be utilized for the development of multi-epitope NDV vaccines. This approach can provide a safer and more efficient method for NDV immunization. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries BMC Veterinary Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Epitope en_US
dc.subject Fusion en_US
dc.subject Hemagglutinin-neuraminidase en_US
dc.subject Newcastle disease virus en_US
dc.subject Peptide-based vaccine en_US
dc.subject Polymerase en_US
dc.subject.mesh Epítopos
dc.subject.mesh Proteína HN
dc.subject.mesh Virus de la Enfermedad de Newcastle
dc.subject.mesh Vacunas de Subunidad
dc.title Identification and evaluation in-vitro of conserved peptides with high affinity to MHC-I as potential protective epitopes for Newcastle disease virus vaccines en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s12917-023-03726-w
dc.relation.issn 1746-6148


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