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Molecular Characterization of Genital and Extragenital Lesions With the PlexPCR VHS Assay in Patients Diagnosed With Syphilis.

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dc.contributor.author Eguiluz, Maria
dc.contributor.author Qquellon Palacios, Luz Jazmín
dc.contributor.author Vargas Rivera, Silver Keith
dc.contributor.author Reyes Díaz, Edward Michael
dc.contributor.author Konda, Kelika Anne
dc.contributor.author Caceres Palacios, Carlos Fernando
dc.contributor.author Klausner, Jeffrey D.
dc.coverage.spatial Lima, Perú
dc.coverage.spatial Callao, Perú
dc.coverage.spatial Pucallpa, Ucayali, Perú
dc.date.accessioned 2023-12-05T17:48:02Z
dc.date.available 2023-12-05T17:48:02Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/14649
dc.description.abstract BACKGROUND: Syphilis diagnosis relies on immunologic markers and clinical protocols. However, syphilitic lesions can be confused with other genital ulcer diseases. METHODS: Using a PlexPCR VHS assay, we analyzed lesion DNA samples from 87 individuals who were clinically diagnosed with early syphilis infection and had at least 1 positive serologic test result. DNA was detected by the PlexPCR VHS multiplex assay and β-globin genes. RESULTS: Among the participants, 99% (86/87) had a positive rapid treponemal test result. DNA was successfully detected in 91% (79/87) of the lesion samples. PlexPCR VHS identified 5 herpes simplex virus (HSV)/Treponema pallidum coinfections (2 HSV-1 and 3 HSV-2), only T pallidum DNA in 62% (49/79), and only HSV-2 in 12.7% (10/79). While 19% (15/79) were negative for all pathogens, none were varicella zoster virus positive. The PlexPCR VHS had 68.4% agreement with the clinical diagnosis. CONCLUSIONS: Since the PlexPCR VHS detects multiple organisms simultaneously, it can help to confirm actual syphilis and identify other pathogen coinfections or the pathogen causing the ulcer. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Open Forum Infectious Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Clinical diagnosis en_US
dc.subject Genital lesions en_US
dc.subject PlexPCR VHS assay en_US
dc.subject syphilis en_US
dc.subject.mesh Diagnóstico Clínico
dc.subject.mesh Genitales
dc.subject.mesh Herpes Simple
dc.subject.mesh Sífilis
dc.title Molecular Characterization of Genital and Extragenital Lesions With the PlexPCR VHS Assay in Patients Diagnosed With Syphilis. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/ofid/ofad483
dc.relation.issn 2328-8957


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