dc.contributor.author |
Eguiluz, Maria |
|
dc.contributor.author |
Qquellon Palacios, Luz Jazmín |
|
dc.contributor.author |
Vargas Rivera, Silver Keith |
|
dc.contributor.author |
Reyes Díaz, Edward Michael |
|
dc.contributor.author |
Konda, Kelika Anne |
|
dc.contributor.author |
Caceres Palacios, Carlos Fernando |
|
dc.contributor.author |
Klausner, Jeffrey D. |
|
dc.coverage.spatial |
Lima, Perú |
|
dc.coverage.spatial |
Callao, Perú |
|
dc.coverage.spatial |
Pucallpa, Ucayali, Perú |
|
dc.date.accessioned |
2023-12-05T17:48:02Z |
|
dc.date.available |
2023-12-05T17:48:02Z |
|
dc.date.issued |
2023 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/14649 |
|
dc.description.abstract |
BACKGROUND: Syphilis diagnosis relies on immunologic markers and clinical protocols. However, syphilitic lesions can be confused with other genital ulcer diseases. METHODS: Using a PlexPCR VHS assay, we analyzed lesion DNA samples from 87 individuals who were clinically diagnosed with early syphilis infection and had at least 1 positive serologic test result. DNA was detected by the PlexPCR VHS multiplex assay and β-globin genes. RESULTS: Among the participants, 99% (86/87) had a positive rapid treponemal test result. DNA was successfully detected in 91% (79/87) of the lesion samples. PlexPCR VHS identified 5 herpes simplex virus (HSV)/Treponema pallidum coinfections (2 HSV-1 and 3 HSV-2), only T pallidum DNA in 62% (49/79), and only HSV-2 in 12.7% (10/79). While 19% (15/79) were negative for all pathogens, none were varicella zoster virus positive. The PlexPCR VHS had 68.4% agreement with the clinical diagnosis. CONCLUSIONS: Since the PlexPCR VHS detects multiple organisms simultaneously, it can help to confirm actual syphilis and identify other pathogen coinfections or the pathogen causing the ulcer. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Oxford University Press |
|
dc.relation.ispartofseries |
Open Forum Infectious Diseases |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Clinical diagnosis |
en_US |
dc.subject |
Genital lesions |
en_US |
dc.subject |
PlexPCR VHS assay |
en_US |
dc.subject |
syphilis |
en_US |
dc.subject.mesh |
Diagnóstico Clínico |
|
dc.subject.mesh |
Genitales |
|
dc.subject.mesh |
Herpes Simple |
|
dc.subject.mesh |
Sífilis |
|
dc.title |
Molecular Characterization of Genital and Extragenital Lesions With the PlexPCR VHS Assay in Patients Diagnosed With Syphilis. |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1093/ofid/ofad483 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
|
dc.relation.issn |
2328-8957 |
|