Universidad Peruana Cayetano Heredia

Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi

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dc.contributor.author Andrade, Alice O.
dc.contributor.author Santos, Najara Akira C.
dc.contributor.author Bastos, Alessandra S.
dc.contributor.author Pontual, José Daniel C.
dc.contributor.author Araújo, Cristiane S.
dc.contributor.author Lima, Analice S.
dc.contributor.author Martinez, Leandro N.
dc.contributor.author Ferreira, Amália S.
dc.contributor.author Aguiar, Anna Caroline C.
dc.contributor.author Teles, Carolina B. G.
dc.contributor.author Guido, Rafael V. C.
dc.contributor.author Santana, Rosa A.
dc.contributor.author Lopes, Stefanie C. P.
dc.contributor.author Medeiros, Jansen F.
dc.contributor.author Rizopoulos, Zaira
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Campo, Brice
dc.contributor.author Lacerda, Marcus Vinicius G.
dc.contributor.author Araújo, Maisa S.
dc.date.accessioned 2023-12-05T17:48:03Z
dc.date.available 2023-12-05T17:48:03Z
dc.date.issued 2023
dc.identifier.uri https://hdl.handle.net/20.500.12866/14658
dc.description.abstract Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A simple centrifugation method appears to be a possible tool for rapidly obtaining purified sporozoites with a minimal loss of yield. However, this method needs to be better defined since sporozoite viability and hepatocyte infection were not evaluated. en_US
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartofseries Scientific Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Parasite biology en_US
dc.subject Parasitology en_US
dc.subject.mesh Parásitos
dc.subject.mesh Parasitología
dc.title Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41598-023-44556-y
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 2045-2322


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