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A novel inexpensive electrochemical sensor for pyrazinoic acid as a potential tool for the identification of pyrazinamide-resistant Mycobacterium tuberculosis

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dc.contributor.author Rueda, Daniel
dc.contributor.author Furukawa, Roberto
dc.contributor.author Fuentes, Patricia
dc.contributor.author Comina, Germán
dc.contributor.author Rey De Castro, Nicolás G.
dc.contributor.author Requena, David
dc.contributor.author Gilman, Robert H.
dc.contributor.author Sheen, Patricia
dc.contributor.author Zimic, Mirko
dc.date.accessioned 2018-11-30T02:09:28Z
dc.date.available 2018-11-30T02:09:28Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/3986
dc.description.abstract Introduction: Tuberculosis (TB) is a significant cause of morbidity and mortality worldwide. The patient compliance with the long treatment regimens is essential for successful eradication. Pyrazinamide (PZA) shortens these regimens from 9 to 6 months, and therefore, improves treatment completion rates. Although PZA is a first-line medication for the treatment of TB, no simple or reliable assay to determine PZA resistance is yet available. In the presence of PZA, only susceptible Mycobacterium tuberculosis strains release pyrazinoic acid (POA). Therefore, the measurement and quantification of released POA is an indicator of PZA resistance. Methods: Two electrochemical sensors were constructed and tested with alternative working electrodes in conjunction with a portable potentiostat to measure the current produced when a potential difference of 2 V is applied to varying concentrations of POA in controlled solutions. Results: The large (13.2 mm) electrochemical sensor was able to detect POA at a minimum concentration of 40 μM to a statistically significant level (P = 0.0190). Similar graphical trends were obtained when testing the electrochemical sensor in the supernatant of a negative microscopic observation drug susceptibility assay culture, irrespective of the presence of PZA. Conclusion: Inexpensive and reusable electrochemical sensors with a portable potentiostat are a promising tool for the detection of POA, a biomarker of PZA susceptible M. Tuberculosis. en_US
dc.language.iso eng
dc.publisher Medknow Publications
dc.relation.ispartof urn:issn:2212-554X
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Electrochemical sensor en_US
dc.subject pyrazinamide en_US
dc.subject pyrazinoic acid en_US
dc.subject resistance en_US
dc.subject tuberculosis en_US
dc.title A novel inexpensive electrochemical sensor for pyrazinoic acid as a potential tool for the identification of pyrazinamide-resistant Mycobacterium tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4103/ijmy.ijmy_63_18
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01


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