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Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon

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dc.contributor.author Moreno, Marta
dc.contributor.author Tong-Rios, Carlos
dc.contributor.author Orjuela-Sanchez, Pamela
dc.contributor.author Carrasco Escobar, Gabriel
dc.contributor.author Campo, Brice
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Winzeler, Elizabeth A.
dc.contributor.author Vinetz, Joseph Michael
dc.date.accessioned 2018-11-30T02:09:31Z
dc.date.available 2018-11-30T02:09:31Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4030
dc.description.abstract In vitro culture of Plasmodium vivax liver stages underlies key understandings of the fundamental biology of this parasite, particularly the latent, hyponozoite stage, toward drug and vaccine development. Here, we report systematic production of Plasmodium vivax sporozoites in colonized Anopheles darlingi mosquitoes in the Peruvian Amazon. Human subject-derived P. vivax-infected blood was fed to Anopheles darlingi females using standard membrane feedings assays. Optimizing A. darlingi infection and sporozoite production included replacement of infected patient donor serum with naïve donor serum, comparing anticoagulants in processing blood samples, and addition of penicillin-streptomycin and ATP to infectious blood meals. Replacement of donor serum by naïve serum in the P. vivax donor blood increased oocysts in the mosquito midgut, and heparin, as anticoagulant, was associated with the highest sporozoite yields. Maintaining blood-fed mosquitoes on penicillin-streptomycin in sugar significantly extended mosquito survival which enabled greater sporozoite yield. In this study, we have shown that a robust P. vivax sporozoite production is feasible in a malaria-endemic setting where infected subjects and a stable A. darlingi colony are brought together, with optimized laboratory conditions. en_US
dc.language.iso eng
dc.publisher American Chemical Society
dc.relation.ispartofseries ACS Infectious Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Anopheles darlingi en_US
dc.subject Plasmodium vivax en_US
dc.subject sporozoite en_US
dc.subject membrane feeding assays en_US
dc.subject Peruvian Amazon en_US
dc.title Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1021/acsinfecdis.7b00195
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 2373-8227


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