Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon

Vinetz, Joseph Michael; Tong-Rios, Carlos; Orjuela-Sanchez, Pamela; Winzeler, Elizabeth A.; Moreno, Marta; Carrasco Escobar, Gabriel; Campo, Brice; Gamboa Vilela, Dionicia Baziliza

dc.contributor.author	Moreno, Marta
dc.contributor.author	Tong-Rios, Carlos
dc.contributor.author	Orjuela-Sanchez, Pamela
dc.contributor.author	Carrasco Escobar, Gabriel
dc.contributor.author	Campo, Brice
dc.contributor.author	Gamboa Vilela, Dionicia Baziliza
dc.contributor.author	Winzeler, Elizabeth A.
dc.contributor.author	Vinetz, Joseph Michael
dc.date.accessioned	2018-11-30T02:09:31Z
dc.date.available	2018-11-30T02:09:31Z
dc.date.issued	2018
dc.identifier.uri	https://hdl.handle.net/20.500.12866/4030
dc.description.abstract	In vitro culture of Plasmodium vivax liver stages underlies key understandings of the fundamental biology of this parasite, particularly the latent, hyponozoite stage, toward drug and vaccine development. Here, we report systematic production of Plasmodium vivax sporozoites in colonized Anopheles darlingi mosquitoes in the Peruvian Amazon. Human subject-derived P. vivax-infected blood was fed to Anopheles darlingi females using standard membrane feedings assays. Optimizing A. darlingi infection and sporozoite production included replacement of infected patient donor serum with naïve donor serum, comparing anticoagulants in processing blood samples, and addition of penicillin-streptomycin and ATP to infectious blood meals. Replacement of donor serum by naïve serum in the P. vivax donor blood increased oocysts in the mosquito midgut, and heparin, as anticoagulant, was associated with the highest sporozoite yields. Maintaining blood-fed mosquitoes on penicillin-streptomycin in sugar significantly extended mosquito survival which enabled greater sporozoite yield. In this study, we have shown that a robust P. vivax sporozoite production is feasible in a malaria-endemic setting where infected subjects and a stable A. darlingi colony are brought together, with optimized laboratory conditions.	en_US
dc.language.iso	eng
dc.publisher	American Chemical Society
dc.relation.ispartofseries	ACS Infectious Diseases
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Anopheles darlingi	en_US
dc.subject	Plasmodium vivax	en_US
dc.subject	sporozoite	en_US
dc.subject	membrane feeding assays	en_US
dc.subject	Peruvian Amazon	en_US
dc.title	Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1021/acsinfecdis.7b00195
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn	2373-8227