Universidad Peruana Cayetano Heredia

Field evaluation of a smartphone-based electronic reader of rapid dual HIV and syphilis point-of-care immunoassays

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dc.contributor.author Allan-Blitz, Lao-Tzu
dc.contributor.author Vargas Rivera, Silver Keith
dc.contributor.author Konda, Kelika A.
dc.contributor.author Herbst de Cortina, Sasha
dc.contributor.author Caceres Palacios, Carlos Fernando
dc.contributor.author Klausner, Jeffrey D.
dc.date.accessioned 2018-11-30T03:10:46Z
dc.date.available 2018-11-30T03:10:46Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4072
dc.description.abstract OBJECTIVE: Electronic (E) devices read and quantify lateral flow-based rapid tests, providing a novel approach to assay interpretation. We evaluated the performance of one E-reader for two dual HIV and syphilis immunoassays. METHODS: We enrolled men who have sex with men and transgender women >18 years of age seeking medical services at an STD clinic in Lima, Peru, between October 2016 and April 2017. Venous blood was tested using two dual HIV and syphilis antibody immunoassays (SD BIOLINE HIV/Syphilis Duo, Republic of Korea, and First Response HIV 1+2/Syphilis Combo, India). Reference testing included a fourth-generation ELISA for HIV antibodies and use of the Treponema pallidum particle agglutination assay for syphilis antibodies. Trained clinic staff visually inspected the immunoassay results, after which the immunoassays were read by the HRDR-200 E-reader (Cellmic, USA), an optomechanical smartphone attachment. We calculated the concordance of the E-reader with visual inspection, as well as the sensitivity of both rapid immunoassays, in detecting HIV and T. pallidum antibodies. RESULTS: On reference testing of 283 participant specimens, 34% had HIV antibodies and 46% had T. pallidum antibodies. Using First Response, the concordance of the E-reader with visual inspection was 97% (95% CI 94% to 99%) for T. pallidum and 97% (95% CI 95% to 99%) for HIV antibodies. Using SD BIOLINE, the concordance of the E-reader with visual inspection was 97% (95% CI 94% to 99%) for T. pallidum and 99% (95% CI 98% to 99%) for HIV antibodies. For both immunoassays, the sensitivity for HIV antibodies was 98% (95% CI 93% to 100%) and the sensitivity for T. pallidum antibodies was 81% (95% CI 73% to 87%). CONCLUSIONS: E-reader results correlated well with visual inspection. The sensitivities of both rapid assays were comparable with past reports. Further evaluation of the E-reader is warranted to investigate its utility in data collection, monitoring and documentation of immunoassay results. en_US
dc.language.iso eng
dc.publisher BMJ Publishing Group
dc.relation.ispartofseries Sexually Transmitted Infections
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject HIV en_US
dc.subject diagnosis en_US
dc.subject syphilis en_US
dc.title Field evaluation of a smartphone-based electronic reader of rapid dual HIV and syphilis point-of-care immunoassays en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1136/sextrans-2017-053511
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.15
dc.relation.issn 1472-3263


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